大麦抗赤霉病近等基因系的转录组分析  

Transcriptome analysis of barley FHB-resistance near-isogenic lines

作　　者：石玲 许开瑞 任圆媛 朱娟 郭宝健[1] 王菲菲 许如根[1] 吕超[1] SHI Ling;XU Kairui;REN Yuanyuan;ZHU Juan;GUO Baojian;WANG Feifei;XU Rugen;LÜChao(Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/College of Agriculture,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学农学院/江苏省作物基因组学与分子育种重点实验室/植物功能基因组学教育部重点实验室,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2024年第5期12-19,64,共9页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家SRT计划国家大麦青稞产业技术体系建设专项(CARS-05);江苏高校优势学科建设工程项目(PAPD)。

摘　　要：赤霉病是大麦主要病害之一,严重影响大麦产量与品质。为研究大麦赤霉病抗性分子机制,以2个大麦赤霉病抗、感近等基因系N53和T66为材料,利用转录组测序技术(RNA-Seq),分析N53、T66在接种禾谷镰刀菌后不同时间点的基因表达特征和差异。结果表明:以接种0 h作为对照,N53和T66分别鉴定出15230和15106个差异表达基因。不同接种时间点上、下调的基因数目和富集到GO及KEGG通路的基因数目在两材料间存在显著差异。不同处理组间共筛选出421个共同差异表达基因,GO功能注释分类分成3大类42个亚类,KEGG通路共涉及到21个,并在信号转导(signal transduction)、植物-病原体相互作用(plant-pathogen interaction)、植物激素信号转导(plant hormone signal transduction)、MAPK-信号通路-植物(MAPK signaling pathway-plant)、苯丙烷类生物合成(phenylpropanoid biosynthesis)通路显著富集。综上,这一研究从转录组水平初步探索大麦对赤霉病的响应机制,可为进一步挖掘大麦赤霉病抗性候选基因、明晰大麦赤霉病抗性分子机制提供参考。Fusarium head blight(FHB)is one of the main diseases of barley,which seriously affects the yield and quality of barley.In order to explore the molecular mechanism of barley FHB resistance,two barley near-isogenic lines N53(resistant)and T66(susceptible)were used as the research objects.Transcriptome sequencing was performed to analyze the differentially expressed gene(DEG)between the two materials at different time points after inoculation with Fusarium graminis.The results showed that 15230 and 15106 differentially expressed genes were identified by N53 and T66,respectively.There were significant differences in the number of up-and down-regulated genes between the two materials at different time points of inoculation,as well as the number of genes enriched in GO and KEGG pathways.A total of 421 common DEG were screened and classified into 3 categories and 42 subclasses by GO functional annotation.KEGG pathway involved a total of 21 pathways.In signal transduction,plant-pathogen interaction,plant hormone signal transduction,MAPK signaling pathway and phenylpropanoid biosynthesis pathway were significantly enriched.This study preliminarily explored the response mechanism of barley to FHB at transcriptomic level,and provided a basis for screening candidate genes and clarifying the molecular mechanism of FHB resistance in barley.

关 键 词：大麦 赤霉病 近等基因系 转录组分析 差异表达基因 

分 类 号：S512.3[农业科学—作物学]