神经介素B及其受体在兔圆小囊中的表达和分布  

The expression and distribution of neuromedin B and its receptor in the rabbit sacculus rotundus

作　　者：李佳[1] 于畅 李雯倩 陈广振 李红霞 王丹丹 张金龙[1,2] 马志禹 LI Jia;YU Chang;LI Wenqian;CHEN Guangzhen;LI Hongxia;WANG Dandan;ZHANG Jinlong;MA Zhiyu(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]扬州大学江苏高校动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2024年第5期90-97,共8页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(31802149);江苏高校优势学科建设工程项目(PAPD)。

摘　　要：为检测神经介素B(neuromedin B,NMB)及其受体(neuromedin B receptor,NMBR)基因和蛋白在圆小囊(sacculus rotundus,SR)中的表达模式和分布情况,制备圆小囊组织石蜡切片,通过HE染色,观察圆小囊组织形态;提取圆小囊组织mRNA,采用实时荧光定量PCR(qPCR)检测NMB和NMBR基因在圆小囊中的表达情况;提取圆小囊组织蛋白,采用免疫印迹(Western blot)检测NMB和NMBR蛋白在圆小囊中的表达情况;并采用免疫组织化学法(IHC)检测NMB和NMBR蛋白在圆小囊中的分布情况。结果表明:圆小囊结构与普通动物肠道类似,但具有发达的淋巴组织,且淋巴组织中散在分布着弥散神经内分泌系统细胞;NMB和NMBR mRNA在不同性别兔的圆小囊中均有表达,但NMBR mRNA在公兔圆小囊中的表达显著高于母兔;NMB和NMBR蛋白在不同性别兔的圆小囊中均有表达,但两者在公兔圆小囊中的表达均显著高于母兔;NMB和NMBR蛋白主要分布于圆小囊的淋巴组织及绒毛内,分布情况略有不同。综上,这一研究从形态学和分子生物学方面明确了NMB及其受体在圆小囊中的表达和分布情况,可为后续深入研究NMB及其受体在圆小囊内的功能奠定基础。To investigate the expression manner and distribution of the neuromedin B(NMB)and neuromedin B receptor(NMBR)gene and protein in the rabbit sacculus rotundus(SR).This study prepared SR tissue sections and stained them with hematoxylin and eosin(HE)to observe the SR tissue morphology.The SR tissue mRNA was extracted and the expression of NMB and NMBR genes was detected by quantitative real-time PCR(qPCR).The SR tissue protein was extracted and the expression of NMB and NMBR proteins was detected by Western blot.Furthermore,the distribution of NMB and NMBR proteins in the SR was detected by immunohistochemistry(IHC).The results showed that the SR structure was similar to that of the normal intestine,but had a well-developed lymphatic tissue,with scattered diffuse neuroendocrine system cells in the lymphatic tissue.NMB and NMBR mRNA were expressed in both male and female rabbit SR,but the expression of NMBR mRNA was significantly higher in male SR than that in female SR.NMB and NMBR proteins were expressed in both male and female rabbit SR,but the expression of both proteins was significantly higher in male SR than that in female SR.NMB and NMBR proteins were mainly distributed in the lymphatic tissue and the villi of the SR,with slightly different distributions.This study clearly identified the expression and distribution of NMB and its receptor in the rabbit SR from both morphological and molecular biological perspectives,laying a foundation for further in-depth research into the functions of NMB and its receptor in the SR.

关 键 词：兔 神经介素B 神经介素B受体 圆小囊 表达 分布 

分 类 号：S829.1[农业科学—畜牧学]