Heterologous expression of the maize transcription factor ZmbHLH36 enhances abiotic stress tolerance in Arabidopsis  

作　　者：Zhenggang Dai Keyong Zhao Dengyu Zheng Siyu Guo Huawen Zou Zhongyi Wu Chun Zhang 

机构地区：[1]College of Agriculture,Yangtze University,Jingzhou 434025,China [2]Institute of Biotechnology,Beijing Academy of Agriculture and Forestry Sciences,Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology,Beijing 100097,China

出　　处：《aBIOTECH》2024年第3期339-350,共12页生物技术通报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.32001430,32171952,31971839).

摘　　要：Basic helix-loop-helix(bHLH)transcription factors are widely distributed in eukaryotes,and in plants,they regulate many biological processes,such as cell differentiation,development,metabolism,and stress responses.Few studies have focused on the roles of bHLH transcription factors in regulating growth,development,and stress responses in maize(Zea mays),even though such information would greatly benefit maize breeding programs.In this study,we cloned the maize transcription factor gene ZmbHLH36(Gene ID:100193615,GRMZM2G008691).ZmbHLH36 possesses conserved domains characteristic of the bHLH family.RT-qPCR analysis revealed that ZmbHLH36 was expressed at the highest level in maize roots and exhibited different expression patterns under various abiotic stress conditions.Transgenic Arabidopsis(Arabidopsis thaliana)plants heterologously expressing ZmbHLH36 had significantly longer roots than the corresponding non-transgenic plants under 0.1 and 0.15 mol L^(−1) NaCl treatment as well as 0.2 mol L^(−1) mannitol treatment.Phenotypic analysis of soil-grown plants under stress showed that transgenic Arabidopsis plants harboring ZmbHLH36 exhibited significantly enhanced drought tolerance and salt tolerance compared to the corresponding non-transgenic plants.Malondialdehyde contents were lower and peroxidase activity was higher in ZmbHLH36-expressing Arabidopsis plants than in the corresponding non-transgenic plants.ZmbHLH36 localized to the nucleus when expressed in maize protoplasts.This study provides a systematic analysis of the effects of ZmbHLH36 on root growth,development,and stress responses in transgenic Arabidopsis,laying a foundation for further analysis of its roles and molecular mechanisms in maize.

关 键 词：Heterologous expression MAIZE Root system Stress responses ZmbHLH36 

分 类 号：Q945.78[生物学—植物学]