猪胚胎冷冻保存的研究进展  

Advances in Cryopreservation of Porcine Embryo

作　　者：董建华 冯肖艺 杨柏高 李崇阳[1] 潘红梅[2] 吕丽华 赵学明[1] DONG Jianhua;FENG Xiaoyi;YANG Baigao;LI Chongyang;PAN Hongmei;L Lihua;ZHAO Xueming(Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Chongqing Academy of Animal Science,Rongchang 402460;College of Animal Science,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]重庆市畜牧科学院,荣昌402460 [3]山西农业大学动物科学学院,太谷030801

出　　处：《畜牧兽医学报》2024年第11期4796-4807,共12页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：重庆地方猪胚胎冷冻保存技术研究与猪育种新材料创制(2022-CHQ-01-01);国家家养动物种质资源库;中国农业科学院科技创新工程(ASTIP-IAS06)。

摘　　要：胚胎冷冻保存对于胚胎的远距离移植和遗传资源的保护具有重要意义。然而,猪胚胎由于其胞质脂肪含量高,对低温敏感,增加了其保存难度。研究表明,通过降低脂质含量、优化培养基成分、保护细胞骨架以及恢复线粒体功能等方式,能够提高猪胚胎冷冻保存技术的效率,从而促进其广泛应用。因此,本文介绍了胚胎冷冻保存技术,总结了提高胚胎冷冻效率的多种方法和措施。同时,笔者团队将通过讨论猪胚胎的内在特性以及低温保存对转录组改变的影响,进一步关注猪胚胎低温保存的机制,从而更好地了解和推进猪胚胎低温保存的研究。Cryopreservation of embryos is important for long-distance embryo transfer and conservation of genetic resources.However,porcine embryos are sensitive to low temperatures due to their high cytoplasmic lipid content,which increases the difficulty of their preservation.Studies have indicated that techniques such as reducing lipid content and optimizing medium composition can significantly enhance the efficiency of porcine embryo cryopreservation.Additionally,protecting the cytoskeleton and restoring mitochondrial function are also crucial strategies that contribute to this advancement.These improvements collectively foster the broader application and sustainable development of this critical technology.Therefore,this article introduces embryo cryopreservation and summaries multiple methods and measures to improve embryo freezing efficiency.Meanwhile,the writers’team will focus more on the mechanism of cryopreservation of porcine embryos by discussing the intrinsic properties of embryos and the effects of cryopreservation on transcriptome alteration to help people better understand and advance research on porcine embryo cryopreservation.

关 键 词：猪 胚胎 冷冻保存 玻璃化冷冻 

分 类 号：S828.3[农业科学—畜牧学]