流式病毒检测法的应用进展  

Application Progress in Flow Virometry

作　　者：卿霆 欧阳和昊 潘其聪 朱碧波[1] 叶静[1] 曹胜波[1] 王秀羽 司有辉[1] QING Ting;OUYANG Hehao;PAN Qicong;ZHU Bibo;YE Jing;CAO Shengbo;WANG Xiuyu;SI Youhui(National Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学农业微生物资源发掘与利用全国重点实验室,武汉430070

出　　处：《畜牧兽医学报》2024年第11期4840-4851,共12页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：“十四五”国家重点研发计划(2021YFD1801105);国家自然科学基金(32373026);中央高校基本科研业务费(2662023DKPY004)。

摘　　要：流式病毒检测法(flow virometry,FVM)是一种利用流式细胞仪检测单个病毒颗粒及其特征的前沿技术。通过FVM,可以精确测量样本中完整病毒颗粒的浓度、表面靶抗原的丰富度以及相对直径等关键参数,进而实现对单个病毒颗粒的高效分析和表征。随着仪器设备、荧光染料和标记策略的不断发展和优化,FVM得到了广泛的应用和研究。该方法不仅被用于实现对单个病毒颗粒的精确分析,还被用于细胞外泌体和微囊泡的检测。本文总结了FVM的发展历程以及病毒等微颗粒的常用标记方法和应用领域,旨在为FVM在病毒学、免疫学、生物医学等研究领域的进一步应用提供参考和借鉴。Flow virometry(FVM)represents a cutting-edge technological approach leveraging flow cytometry for the precise detection and analysis of individual virus particles and their unique characteristics.This innovative method offers a novel means of detecting,quantifying,and characterizing individual virus particles.FVM enables the determination of various parameters including the concentration of intact virus particles,the abundance of specific target antigens present on the virus surface,and the relative diameter of the viruses.Within the field of technique,FVM extends beyond virus analysis,allowing for the effective detection and characterization of exosomes and microvesicles originating from cells.With the continuous development and optimization of instruments,fluorescent dyes and labeling strategies,FVM has been widely used and studied.This paper summarizes the development history of FVM,as well as the common labeling methods and application fields of viruses and other microparticles,aiming to provide reference for the further application of FVM in virology,immunology,biomedics and other research fields.

关 键 词：流式病毒检测法 流式细胞术 病毒和外泌体标记 

分 类 号：R392-33[医药卫生—免疫学] S859.797[医药卫生—基础医学]