免疫层析技术在猪常见病毒病快速检测中的应用  

Application of Immunochromatographic Technique in the Rapid Detection of Common Swine Viral Diseases

作　　者：芦烘德 刘昊阳 龚诗淼 杨植 王煜轩 王禄皓 何至远 董虹[1,2] LU Hongde;LIU Haoyang;GONG Shimiao;YANG Zhi;WANG Yuxuan;WANG Luhao;HE Zhiyuan;DONG Hong(Beijing Key Laboratory of TCVM,Beijing University of Agriculture,Beijing 102206,China;Beijing Engineering Research Center of CVM,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]北京农学院兽医学(中医药)北京市重点实验室,北京102206 [2]北京农学院北京市中兽药工程技术研究中心,北京102206

出　　处：《畜牧兽医学报》2024年第11期4900-4911,共12页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：“十四五”重点研发项目(2022YFD1801103);国家自然科学基金项目(32302916);家畜产业技术体系北京市家畜创新团队(BAIC05-2023);北京农学院青年教师科研创新能力提升计划资助项目(QJKC-2023011);北京农学院科技创新“火花行动”支持计划(BUA-HHXD2023001);市属高校分类发展都市农林特色教师队伍建设(11000024T000002961733)。

摘　　要：免疫层析技术是一类典型的以抗原抗体特异性免疫反应为基础的快速诊断技术,以不同材料作为示踪标记物,具有快速、特异、价格低廉的特点,对猪常见病毒病的现场检测具有广泛的应用价值。本文基于免疫层析技术原理,根据不同的信号表现方式将当前常见的免疫层析技术进行了归类,详细介绍了比色型免疫层析技术,荧光型免疫层析技术的代表性标志物,综述了免疫层析技术在猪常见病毒病快速检测中的应用,并将不同标记物免疫层析试纸条的优点与挑战进行比较,以期为猪病毒病快速检测技术的发展提供思路。Immunochromatography is a typical rapid diagnostic technology based on antigen-antibody-specific immune reaction,using different materials as tracer markers,which is rapid,specific and inexpensive,and has wide application value for on-site detection of common viral diseases in pigs.In this paper,based on the principle of immunochromatographic technology,the current common immunochromatographic techniques were categorized according to different signal manifestation modes,colorimetric immunochromatographic techniques,fluorescent immunochromatographic techniques of the representative markers were introduced in detail,the application of immunochromatographic techniques in the rapid detection of common porcine viral diseases was reviewed,and the advantages and challenges of immunochromatographic test strips with different markers were compared,with a view to providing ideas for the development of rapid detection techniques for porcine viral diseases.

关 键 词：免疫层析技术 猪病毒病 快速检测 临床应用 

分 类 号：S854.4[农业科学—临床兽医学]