转录组和蛋白组筛选就巢鸡卵巢发育候选基因及其调控网络构建  

Screening Candidate Genes for Ovarian Development and Constructing Regulatory Network in Nesting Chickens by Transcriptome and Proteome

作　　者：蒋婷[1] 李文东 李兴起 黄雨 王启贵 王海威 杨朝武[3] 刘凌斌 JIANG Ting;LI Wendong;LI Xingqi;HUANG Yu;WANG Qigui;WANG Haiwei;YANG Chaowu;LIU Lingbin(College of Animal Science and Technology,Southwest University,Chongqing 400715,China;Chongqing Academy of Animal Sciences,Chongqing 402460,China;Sichuan Animal Science Academy,Chengdu 610066,China)

机构地区：[1]西南大学动物科学技术学院,重庆400715 [2]重庆市畜牧科学研究院,重庆402460 [3]四川省畜牧科学研究院,成都610066

出　　处：《畜牧兽医学报》2024年第11期4950-4967,共18页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：重庆市研究生科研创新项目(CYS21126);重庆市技术创新与应用发展专项重点项目(CSTB2023TIAD-LUX0003);西南大学大学生创新创业训练计划项目(202310635032)。

摘　　要：为了研究蛋鸡就巢性的潜在调控机制,本研究选用300日龄体况良好、体重一致的城口山地鸡12只,其中3只个体处于正常产蛋期,其余9只个体分别处于就巢期10、20和30天。采集各组蛋鸡卵巢组织进行组织解剖学形态观察,然后利用转录组(RNA-seq)和蛋白组(iTRAQ)测序技术分别对产蛋组正常卵巢(normal ovary,NO)和就巢组萎缩卵巢(atrophic ovary,AO)进行测序分析。筛选差异表达基因(DEGs)和差异表达蛋白(DEPs)并进行功能富集分析,鉴定与家禽就巢性相关的候选基因。结果表明:就巢导致蛋鸡卵巢发生萎缩,卵泡大量闭锁。在卵巢组织中鉴定出930个DEGs,其中430个基因上调,500个基因下调;同时鉴定出546个DEPs,其中178个蛋白上调,368个蛋白下调。通过功能注释和富集分析发现,这些DEGs和DEPs显著富集在细胞外基质(extracellular matrix,ECM)受体相互作用、黏着和PI3K-Akt等信号通路。最后通过转录组和蛋白组联合分析,筛选出7个蛋鸡就巢性的候选基因:COMP、FN1、ITGA8、THBS1、COL4A2、COL4A1和COL1A1。综上所述,本研究通过分析转录组测序和蛋白组测序结果筛选了蛋鸡就巢性关键候选基因,并构建了家禽就巢状态下卵巢发育的调控网络。本研究为深入解析就巢期卵巢萎缩的分子调控机制提供了理论参考,丰富了调控家禽就巢性状的候选基因,为蛋鸡的遗传改良和分子育种研究提供理论依据。The study aimed to investigate the potential regulatory mechanism of nesting ability of laying hens.The 12300-day-old Chengkou mountain chickens with good body condition and the same body weight were used in this study.Among them,3 individuals were in normal laying period,and the remaining 9 individuals were in nesting period for 10,20 and 30 days,respectively.The ovarian tissues of laying hens in each group were collected for anatomical observation.Normal ovary(NO)and atrophic ovary(AO)of laying hens were analyzed by RNA-seq and iTRAQ sequencing technology.Differentially expressed genes(DEGs)and differentially expressed proteins(DEPs)were screened and functional enrichment analysis was performed to identify candidate genes related to nesting in poultry.The results showed that ovary atrophy and follicle atresia occurred in nesting hens.A total of 930 DEGs were identified in ovarian tissues,of which 430 genes were up-regulated and 500 genes were down-regulated.Meanwhile,546 DEPs were identified,of which 178 proteins were up-regulated and 368 proteins were down-regulated.Through functional annotation and enrichment analysis,it was found that these DEGs and DEPs were significantly enriched in ECM receptor interaction,adhesion and PI3K-Akt signaling pathways.Finally,7 candidate genes for nesting ability COMP,FN1,ITGA8,THBS1,COL 4A2,COL 4A1 and COL 1A1 were selected by combined transcriptome and proteome analysis.In summary,key candidate genes for nesting ability were identified by transcriptome and proteome analysis,and a regulatory network for ovarian development in nesting poultry was constructed.This study provides a theoretical reference for further understanding the molecular regulation mechanism of ovarian atrophy at nesting stage,enriches the candidate genes regulating poultry nesting traits,and provides theoretical basis for genetic improvement and molecular breeding research of laying hens.

关 键 词：就巢 卵巢 转录组学 蛋白组学 城口山地鸡 

分 类 号：S831.2[农业科学—畜牧学]