犬冬眠心肌跨室壁三层细胞的电生理记录  

Electrophysiological records of three layers of transventricular cells in hibernating myocardium in beagles

作　　者：高万里 宋昆鹏 杨红晓 李艳 臧小彪 王现青 高传玉 赵永辉 Gao Wanli;Song Kunpeng;Yang Hongxiao;Li Yan;Zang Xiaobiao;Wang Xianqing;Gao Chuanyu;Zhao Yonghui(Department of Cardiology,Zhengzhou Central Hospital,Zheng zhou University,Zheng zhou 450007,China;不详)

机构地区：[1]郑州大学附属郑州中心医院心内科,郑州450007 [2]阜外华中心血管病医院心内科,郑州450003

出　　处：《中国循证心血管医学杂志》2024年第10期1208-1211,共4页Chinese Journal of Evidence-Based Cardiovascular Medicine

基　　金：河南省科技厅基础与前沿科技攻关计划(12230041013)。

摘　　要：目的记录跨室壁三层细胞离子流在正常和冬眠心肌中的差异表达,进一步探索其与心源性猝死的关系。方法采用同期全细胞膜片钳技术测定酶解分离的犬冬眠心肌外、中、内层细胞膜离子流慢激活延迟整流钾电流(简称IKs)、动作电位时程(简称APD),由此计算其跨室壁复极离散(TDR)。结果与正常心肌组(以下简称B组)相比,犬冬眠心肌组(简称A组)跨室壁三层细胞的IKs减小,APD延长,且以中层心肌细胞为著;与B组相比,A组TDR明显增加。结论心肌冬眠时,三层心肌细胞的IKs明显减小,APD延长,且心肌细胞的这种变化呈现不均一性,进一步增加了TDR,为恶性心律失常的发生提供了电生理基础,可能使冬眠心肌成为心源性猝死的一个独立危险因素。Objective To record differential expressions of three-layer transventricular ion flows in normal and hibernating myocardium,and further explore the relationship among IKs,APD and sudden cardiac death(SCD).Methods The potassium ion current(IKs)and action potential duration(APD)of cytomembrane in epicardium,midmyocardium and endocardium in Beagle hibernating myocardium isolated by enzymatic hydrolysis were detected by using simultaneous whole-cell patch-clip technique,and transmural dispersion of repolarization(TDR)was calculated.Results Compared with normal myocardium group(group B),IKs decreased and APD increased in three-layer transventricular cells,which were more significantly in midmyocardium in hibernating myocardium group(group A).TDR increased significantly in group A compared with group B.Conclusion In hibernating myocardium,IKs decreases significantly and APD increases,and these changes of myocardial cells show heterogeneity.These changes promote TDR,and provide electrophysiological basis for occurrence of malignant arrhythmia,which may be an independent risk factor for SCD.

关 键 词：冬眠心肌 膜片钳 电生理 心源性猝死 

分 类 号：R542.2[医药卫生—心血管疾病]