氨基寡糖素与噻霉酮复配比例筛选及对烟草青枯病的防控效果  

Amino-oligosaccharide and benzothiazolinone compound ratio selection and their control efficacies on tobacco bacterial wilt

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作  者:王垚 梁依佳 杨磊 梁颖涛[3] 汪安斌 马兴隽 丁伟[1] 李石力[1] WANG Yao;LIANG Yijia;YANG Lei;LIANG Yingtao;WANG Anbin;MA Xingjun;DING Wei;LI Shili(College of Plant Protection,Southwest University,Chongqing 400715,China;China Tobacco Hunan Industrial Co.,Ltd.,Changsha 410000,China;Chongqing Branch of CNTC,Chongqing 400023,China;College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China)

机构地区:[1]西南大学植物保护学院,重庆市400715 [2]湖南中烟工业有限责任公司,长沙市410000 [3]中国烟草总公司重庆市公司,重庆市400023 [4]西南大学农学与生物科技学院,重庆市400715

出  处:《烟草科技》2024年第10期59-67,共9页Tobacco Science & Technology

基  金:湖南中烟工业有限责任公司科技计划项目“新品类卷烟核心原料BFO/BFF生产技术体系研究及推广”(KY2020JD0010);中国烟草总公司重庆市公司科技项目“渝东南植烟土壤改良关键技术集成与示范应用”(B20221NY1312)。

摘  要:为筛选出氨基寡糖素与噻霉酮对烟草青枯病防治的最佳复配比例,采用滤纸片法测定了氨基寡糖素与噻霉酮不同质量配比对烟草青枯病菌的毒力,并通过室内盆栽和大田试验研究了最佳复配比例的促生控病效果。结果表明:氨基寡糖素与噻霉酮质量配比(简称复配剂)为1∶3对青枯病菌CQPS-1的毒力最高,且略高于噻霉酮单剂,EC_(50)分别为22.05 mg/L和23.69 mg/L。室内盆栽试验表明,复配剂的防效高于噻霉酮和氨基寡糖素单剂。田间试验表明,两者复配能够改善烟株的农艺性状和促进烟株的根系发育,效果高于氨基寡糖素和噻霉酮单剂。其中,打顶期的株高、茎围、腰叶长、腰叶宽和叶面积均显著高于对照,分别提高10.42%、8.57%、14.93%、10.30%和26.55%。田间防效结果显示,复配剂防治效果高于单剂,且在发病初期(施药后15 d)和发病高峰期(施药后48 d)相对防效分别为78.06%和73.68%,高于噻霉酮单剂的70.54%、64.76%和氨基寡糖素单剂的44.40%和42.05%。因此,氨基寡糖素与噻霉酮复配能提高噻霉酮的抑菌活性与相对防效。To select the optimal compound ratio for amino-oligosaccharide and benzothiazolinone to control tobacco bacterial wilt,the toxicities of different mass proportions of amino-oligosaccharide and benzothiazolinone against R.solanacearum strain CQPS-1 were determined by a filter paper approach.The growth promotion and control efficacy of the optimal compound ratio of amino-oligosaccharide and benzothiazolinone were investigated by indoor pot and field tests.The results showed that when the compound ratio was 1:3,the mixture of amino-oligosaccharide and benzothiazolinone(abbreviated as compounded agent)had the highest toxicity against the strain CQPS-1,which was slightly higher than that of benzothiazolinone,and their EC_(50) were 22.05 and 23.69 mg/L,respectively.The results of the pot experiment showed that the control efficacy of the compounded agent was higher than that of benzothiazolinone or amino-oligosaccharide alone.The results of the field experiment showed that the compounded agent was able to improve the agronomic traits and promote the development of tobacco root,and the growth promoting effect was better than that of amino-oligosaccharide or benzothiazolinone.Compared with the control group,plant height,stalk circumference,middle leaf length,middle leaf width and leaf area at the topping stage were significantly increased by 10.42%,8.57%,14.93%,10.30%and 26.55%,respectively.The control efficacy of the compounded agent was better than that of the single agent on tobacco bacterial wilt.The relative control efficacies of the compounded agent at the early(15 days after application)and peak(48 days after application)stages of infection were 78.06%and 73.68%,respectively,which were higher than those of benzothiazolinone(70.54%and 64.76%)and amino-oligosaccharide(44.40%and 42.05%).Therefore,the combination of amino-oligosaccharide and benzothiazolinone could enhance the antibacterial activity and control effect of benzothiazolinone.

关 键 词:烟草青枯病 噻霉酮 氨基寡糖素 复配剂 增效作用 相对防效 

分 类 号:S482.299[农业科学—农药学]

 

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