Instant trachea reconstruction using 3D-bioprinted C-shape biomimetic trachea based on tissue-specific matrix hydrogels  

作　　者：Yuyan Sun Yingying Huo Xinyue Ran Hongying Chen Qingqing Pan Yujie Chen Ying Zhang Wenjie Ren Xiaoyun Wang Guangdong Zhou Yujie Hua 

机构地区：[1]Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai Key Laboratory of Tissue Engineering,Shanghai,200011,PR China [2]Research Institute of Plastic Surgery,Weifang Medical University,Weifang,Shandong,261053,PR China [3]Department of Plastic Surgery,Tongren Hospital Shanghai Jiao Tong University School of Medicine,Shanghai,200050,PR China [4]Institute of Regenerative Medicine and Orthopedics,Institutes of Health Central Plain,Xinxiang Medical University,Xinxiang,Henan,453003,PR China [5]National Tissue Engineering Center of China,Shanghai,200241,PR China [6]Morphology and Spatial Multi-omics Technology Platform,Shanghai Institute of Nutrition and Health,Chinese Academy of Sciences,Shanghai,200031,PR China

出　　处：《Bioactive Materials》2024年第2期52-65,共14页生物活性材料（英文）

基　　金：financially supported by the National Key Research and Development Program of China(2022YFA1207500);Biomaterials and Regenerative Medicine Institute Cooperative Research Project of Shanghai Jiaotong University School of Medicine(2022LHA07);the National Natural Science Foundation of China(82302823,81871502 and 81671837);the Key Research and Development Program of Henan Province(221111310100);Major Science and Technology Projects of Xinxiang City(21ZD006);Shanghai Municipal Science and Technology Major Project(21Y11911700);Science and Technology Innovation Action Plan Venus Project(Sailing Special Project,23YF1421400);the China Postdoctoral Science Foundation(2023M732294);Shanghai Municipal Key Clinical Specialty(shslczdzk06601).

摘　　要：Currently,3D-bioprinting technique has emerged as a promising strategy to offer native-like tracheal substitutes for segmental trachea reconstruction.However,there has been very limited breakthrough in tracheal repair using 3D-bioprinted biomimetic trachea owing to the lack of ideal bioinks,the requirement for precise structural biomimicking,and the complexity of multi-step surgical procedures by mean of intramuscular pre-implantation.Herein,we propose a one-step surgical technique,namely direct end-to-end anastomosis using C-shape 3D-bioprinted biomimetic trachea,for segmental trachea defect repair.First,two types of tissue-specific matrix hydrogels were exploited to provide mechanical and biological microenvironment conducive to the specific growth ways of cartilage and fibrous tissue respectively.In contrast to our previous O-shape tracheal design,the tubular structure of alternating C-shape cartilage rings and connecting vascularized-fibrous-tissue rings was meticulously designed for rapid 3D-bioprinting of tracheal constructs with optimal printing paths and models.Furthermore,in vivo trachea regeneration in nude mice showed satisfactory mechanical adaptability and efficient physiological regeneration.Finally,in situ segmental trachea reconstruction by direct end-to-end anastomosis in rabbits was successfully achieved using 3D-bioprinted C-shape biomimetic trachea.This study demonstrates the potential of advanced 3D-bioprinting for instant and efficient repair of segmental trachea defects.

关 键 词：Trachea reconstruction 3D-bioprinting HYDROGELS Biomimetic architecture 

分 类 号：R318.1[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]