Full-thickness osteochondral defect repair using a biodegradable bilayered scaffold of porous zinc and chondroitin sulfate hydrogel  

作　　者：Fan Yang Yageng Li Lei Wang Haodong Che Xin Zhang Holger Jahr Luning Wang Dong Jiang Hongjie Huang Jianquan Wang 

机构地区：[1]Department of Sports Medicine,Peking University Third Hospital,Institute of Sports Medicine of Peking University,Beijing Key Laboratory of Sports Injuries,Engineering Research Center of Sports Trauma Treatment Technology and Devices,Ministry of Education,Beijing,China [2]Beijing Advanced Innovation Center for Materials Genome Engineering,State Key Laboratory for Advanced Metals and Materials,School of Materials Science and Engineering,University of Science and Technology Beijing,Beijing,China [3]Institute of Anatomy and Cell Biology,University Hospital RWTH Aachen,Aachen,52074,Germany [4]Institute of Structural Mechanics and Lightweight Design,RWTH Aachen University,52062,Aachen,Germany

出　　处：《Bioactive Materials》2024年第2期400-414,共15页生物活性材料（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(grant numbers:82072403,82072428,82272571,82267020,82372418,52201294);Beijing Natural Science Foundation Haidian Original Innovation Joint Fund Frontier Project(L212052);Beijing Natural Science Foundation(L212014);Prosperos project,funded by the Interreg VA Flanders-The Netherlands program,CCI Grant No.2014TC16RFCB04;the Interdisciplinary Centre for Clinical Research(IZKF)of the Faculty of Medicine of the RWTH Aachen University(OC1-1).

摘　　要：The regeneration of osteochondral tissue necessitates the re-establishment of a gradient owing to the unique characteristics and healing potential of the chondral and osseous phases.As the self-healing capacity of hyaline cartilage is limited,timely mechanical support during neo-cartilage formation is crucial to achieving optimal repair efficacy.In this study,we devised a biodegradable bilayered scaffold,comprising chondroitin sulfate(CS)hydrogel to regenerate chondral tissue and a porous pure zinc(Zn)scaffold for regeneration of the underlying bone as mechanical support for the cartilage layer.The photocured CS hydrogel possessed a compressive strength of 82 kPa,while the porous pure Zn scaffold exhibited a yield strength of 11 MPa and a stiffness of 0.8 GPa.Such mechanical properties are similar to values reported for cancellous bone.In vitro biological experiments demonstrated that the bilayered scaffold displayed favorable cytocompatibility and promoted chondrogenic and osteogenic differentiation of bone marrow stem cells.Upon implantation,the scaffold facilitated the simultaneous regeneration of bone and cartilage tissue in a porcine model,resulting in(i)a smoother cartilage surface,(ii)more hyaline-like cartilage,and(iii)a superior integration into the adjacent host tissue.Our bilayered scaffold exhibits significant potential for clinical application in osteochondral regeneration.

关 键 词：Chondroitin sulfate hydrogel Additive manufacturing Porous Zn scaffold Osteochondral regeneration 

分 类 号：R318.08[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]