Facile and rapid fabrication of a novel 3D-printable,visible light-crosslinkable and bioactive polythiourethane for large-to-massive rotator cuff tendon repair  

作　　者：Xu Zhang Ke Li Chenyang Wang Ying Rao Rocky STuan Dan Michelle Wang Dai Fei Elmer Ker 

机构地区：[1]Institute for Tissue Engineering and Regenerative Medicine,The Chinese University of Hong Kong,New Territories,Hong Kong SAR,China [2]School of Biomedical Sciences,Faculty of Medicine,The Chinese University of Hong Kong,New Territories,Hong Kong SAR,China [3]Center for Neuromusculoskeletal Restorative Medicine,Hong Kong Science Park,Hong Kong SAR,China [4]Ministry of Education Key Laboratory for Regenerative Medicine,School of Biomedical Sciences,Faculty of Medicine,The Chinese University of Hong Kong,New Territories,Hong Kong SAR,China [5]Department of Orthopaedics and Traumatology,Faculty of Medicine,The Chinese University of Hong Kong,New Territories,Hong Kong SAR,China

出　　处：《Bioactive Materials》2024年第7期439-458,共20页生物活性材料（英文）

基　　金：supported by Hong Kong Health Bureau (DFEK:Health Medical and Research Fund,08190466,DMW:Health Medical and Research Fund,07180686);Hong Kong Research Grants Council (DFEK:Early Career Scheme Award,24201720,General Research Fund:14213922,DMW:General Research Fund:14118620 and 14121121);National Natural Science Foundation of China-Hong Kong Research Grants Council Joint Research Scheme (DMW:N_CUHK409/23);Hong Kong Innovation and Technology Commission (DFEK:Tier 3 Award,ITS/090/18,DW:ITS/333/18;DFEK,DMW,and RST:Health@InnoHK program);The Chinese University of Hong Kong (DFEK:Faculty Innovation Award,FIA2018/A/01)。

摘　　要：Facile and rapid 3D fabrication of strong,bioactive materials can address challenges that impede repair of large-to-massive rotator cuff tears including personalized grafts,limited mechanical support,and inadequate tissue regeneration.Herein,we developed a facile and rapid methodology that generates visible light-crosslinkable polythiourethane(PHT)pre-polymer resin(~30 min at room temperature),yielding 3D-printable scaffolds with tendon-like mechanical attributes capable of delivering tenogenic bioactive factors.Ex vivo characterization confirmed successful fabrication,robust human supraspinatus tendon(SST)-like tensile properties(strength:23 MPa,modulus:459 MPa,at least 10,000 physiological loading cycles without failure),excellent suture retention(8.62-fold lower than acellular dermal matrix(ADM)-based clinical graft),slow degradation,and controlled release of fibroblast growth factor-2(FGF-2)and transforming growth factor-β3(TGF-β3).In vitro studies showed cytocompatibility and growth factor-mediated tenogenic-like differentiation of mesenchymal stem cells.In vivo studies demonstrated biocompatibility(3-week mouse subcutaneous implantation)and ability of growth factor-containing scaffolds to notably regenerate at least 1-cm of tendon with native-like biomechanical attributes as uninjured shoulder(8-week,large-to-massive 1-cm gap rabbit rotator cuff injury).This study demonstrates use of a 3D-printable,strong,and bioactive material to provide mechanical support and pro-regenerative cues for challenging injuries such as large-to-massive rotator cuff tears.

关 键 词：Photo-crosslinkable biomaterials POLYURETHANE Click reactions 3D-printing Growth factors Rotator cuff tendon tissue engineering 

分 类 号：R68[医药卫生—骨科学]