防护风镜对眼爆震伤诱导视网膜病理损伤和自噬变化的有效改善  

Effective improvement of retinal pathological injury and autophagy changes induced by explosive eye blast injury by protective goggles

作　　者：晁望舒 费晓炜 李伟萍 豆雅楠 王俊龙 何鑫 吕伟豪 武秀权 陈红庆 陈雷颖 马天 费舟 庄茁[4] 康越 费霏 CHAO Wangshu;FEI Xiaowei;LI Weiping;DOU Ya'nan;WANG Junlong;HE Xin;LYU Weihao;WU Xiuquan;CHEN Hongqing;CHEN Leiying;MA Tian;FEI Zhou;ZHUANG Zhuo;KANG Yue;FEI Fei(Basic Medical Science Academy,Air Force Medical University,Xi'an 710032,Shaanxi,China;Xijing Hospital,Air Force Medical University,Xi'an 710032,Shaanxi,China;Institute of Systems Engineering,Academy of Military Sciences,Beijing 100010,China;School of Aerospace Engineering,Tsinghua University,Beijing 100084,China)

机构地区：[1]空军军医大学基础医学院,陕西西安710032 [2]空军军医大学西京医院,陕西西安710032 [3]军事医学科学院系统工程研究院,北京100010 [4]清华大学航天航空学院,北京100084

出　　处：《爆炸与冲击》2024年第12期159-177,共19页Explosion and Shock Waves

基　　金：陕西省自然科学基础研究计划重点项目(2023-JC-ZD-48);空军军医大学军事医学提升计划(2021JSTS14);西京医院医务人员培养交叉融合专项(XJZT24JC39)。

摘　　要：首先基于头部动态测试系统与激波管和外场实弹实爆测试环境,验证了眼部装备(护目镜和风镜)的防护性能。研究结果表明,风镜防护性能更优,建议给执勤人员配发兼容防紫外、强光、烟雾和防破片功能的风镜产品,以提高相关人员眼部防护能力。此后,研究眼部爆震伤后组织损伤、功能改变及市售风镜动物实验版的防护作用与机制,为防治眼部爆震伤及风镜后续的设计改进提供理论依据。选用比格犬和C57小鼠进行相关动物实验,通过HE、Tunel、Nissl染色、视觉电生理检查等检测方法,发现随着冲击波强度的提高和伤后时间的延长,视网膜损伤程度和细胞凋亡程度均提高,其中神经节细胞层(ganglion cell layer,GCL)和光感受器内外节交界处(layer of photoreceptor inner/outer segments,IS/OS)受到的损伤最严重;进一步研究分子变化发现,自噬相关调节蛋白SQSTM1/p62(P<0.0001)和LC3-Ⅱ(P=0.8437)、LC3-Ⅰ(P=0.003)的表达量明显增高,说明视网膜损伤一定程度上是由爆震伤后自噬增强这一机制导致的。市售风镜的动物实验版能够有效减轻冲击波对视网膜的损伤,保护RNFL、INL/ONL、GCL和IS/OS的结构。同时,与其他组相比,3.5 MPa组防护组与损伤组视网膜损伤和细胞凋亡程度差异最显著,推测该强度下防护风镜发挥了最大的保护作用,保护机制与防护降低视网膜细胞自噬相关。We firstly verified the protective performance of eye equipment(goggles)based on a head dynamic test system and shock tube and field live blast test environments.The results show that goggles have better protective performance and suggest that duty personnel should be equipped with goggles that have combined functions of anti-ultraviolet,anti-glare,anti-smoke and anti-fragmentation in to improve eye protection capabilities.After that,we investigated the tissue damage and functional impairment changes after explosive eye blast injury,and the protective effect and mechanism of the goggles for animal experimental version.This may provide a theoretical basis for prevention and treatment,and also have important implications for the design and improvement of protective goggles.Beagles and C57 mice were used for related animal experiments,and the changes in retinal layer thickness and cell apoptosis were observed after blast injury by HE,Tunel,Nissl staining,visual electrophysiology detection and other methods.It was found that with the increase of blast intensity and the extension of time after explosion,both the degree of retinal injury and cell apoptosis increased,among which the ganglion cell layer and photoreceptor inner and outer segments suffered the most severe damage.Further research on molecular changes indicates that the expression levels of autophagy-related regulatory proteins SQSTM1/p62(P<0.0001)and LC3-Ⅱ(P=0.8437),as well as LC3-Ⅰ(P=0.003),are significantly increased,suggesting that retinal damage is,to some extent,induced by autophagic mechanisms.The protective goggles could effectively reduce the damage of blast wave to retina,protect the structural integrity of retinal nerve fiber layer,inner and outer nuclear layer,ganglion cell layer and photoreceptor inner and outer segments.At the same time,compared with that of other groups,the difference in retinal layer thickness and cell apoptosis was most significant in the 3.5 MPa group,suggesting that the glasses played the maximum protective effect at t

关 键 词：比格犬 眼部爆震伤 防护风镜 视网膜 线粒体 自噬 

分 类 号：O389[理学—流体力学]