草地贪夜蛾的冷识别温度及低温胁迫下Hsp70和Hsp90基因的响应  

Determination of discriminating temperatures for Spodoptera frugiperda and the response of Hsp70 and Hsp90 genes under low temperature stress

作　　者：田彩红[1] 张俊逸 党菁 黄建荣[1] 尹新明[2] 李国平[1] 封洪强[1] TIAN Caihong;ZHANG Junyi;DANG Jing;HUANG Jianrong;YIN Xinming;LI Guoping;FENG Hongqiang(Institute of Plant Protection,Henan Academy of Agricultural Sciences/Henan Key Laboratory of Crop Pest Control/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China,Ministry of Agriculture and Rural Affairs of the People’s Republic of China/international Joint Research Laboratory for Crop Protection of Henan/Biological Pesticides Engineering Research Center of Henan Province,Zhengzhou,Henan 450002;College of Plant Protection/Henan International Laboratory for Green Pest Control,Henan Agricultural University,Zhengzhou,Henan 450002;Yuzhou Science and Technology Bureau,Yuzhou,Xuchang,Henan 461670,China)

机构地区：[1]河南省农业科学院植物保护研究所,河南省农作物病虫害防治重点实验室,农业农村部华北南部作物有害生物综合治理重点实验室,河南省作物保护国际联合实验室,河南省生物农药工程研究中心,郑州450002 [2]河南农业大学植物保护学院,河南省害虫绿色防控国际联合实验室,郑州450002 [3]禹州市科学技术局,河南禹州461670

出　　处：《热带生物学报》2024年第6期664-671,共8页Journal of Tropical Biology

基　　金：国家重点研发计划(2021YFD1400701);河南省科技攻关项目(232103810016);2024年度省级科技研发计划联合基金项目;河南省重大专项(201300111500);河南省农业科学院科技创新团队(2024TD30)。

摘　　要：为了探明草地贪夜蛾(Spodoptera frugiperda)幼虫和蛹的抗寒能力,室内测定了草地贪夜蛾1~6龄幼虫和蛹在低温条件下的死亡率,拟合线性回归方程,统计各发育阶段的冷识别温度,得到1~6龄幼虫和蛹的冷识别温度分别为-7.80℃、-9.70℃、-9.61℃、-8.78℃、-8.29℃、-8.05℃和-9.51℃。通过实时荧光定量PCR对常温饲养和低温处理的草地贪夜蛾的Hsp 70基因(GenBank登录号:NC_064223.1)和Hsp 90基因(GenBank登录号:MN832694)进行表达分析,结果表明2种处理的草地贪夜蛾的Hsp 70基因和Hsp 90基因在不同发育阶段表达量均不同,经过低温处理后,Hsp70基因在1龄幼虫中表达量显著降低(P<0.05),在2~6龄幼虫和蛹中表达量极显著增加(P<0.01);Hsp90基因在1~6龄幼虫中表达量极显著增加(P<0.01),在蛹中表达量极显著降低(P<0.01)。Mortality rates of 1-6 instar larvae and pupae of Spodoptera frugiperda under low temperature conditions were measured in the laboratory to explore the cold resistance of the larvae and pupae of S.frugiperda(J.E.Smith).The linear regression equation was fitted and the discriminating temperatures at each developmental stage were calculated.The discriminating temperatures for 1-6 instar larvae and pupae were-7.80°C,-9.70°C,-9.61°C,-8.78°C,-8.29°C,-8.05°C and-9.51°C,respectively.The expression of Hsp70 gene(GenBank accession number:NC_064223.1)and Hsp90 gene(GenBank accession number:MN832694)of S.frugiperda reared at room temperature and treated at low temperature was analyzed by using quantitative realtime PCR.The results showed that the expression levels of Hsp70 gene and Hsp90 gene of S.frugiperda reared at room temperature and treated at low temperature were different at different developmental stages.After low temperature treatment,the expression level of Hsp70 gene in the first instar larvae was significantly reduced(P<0.05),while the expression levels in 2nd-6th instar larvae and pupae were significantly increased(P<0.01).The expression level of Hsp90 gene in 1st-6th instar larvae was significantly increased(P<0.01),and the expression level in pupae was significantly decreased(P<0.01).

关 键 词：草地贪夜蛾 冷识别温度 HSP70 HSP90 实时荧光定量PCR 

分 类 号：Q966[生物学—昆虫学]