杧果超氧化物歧化酶基因家族鉴定与生物信息学分析  

Identification and bioinformatics analysis of superoxidedismutase gene in Mangifera indica

作　　者：施绍璞 周开兵[1,2] SHI Shaopu;ZHOU Kaibing(School of Breeding and Multiplication/Sanya Institute of Breeding and Multiplication,Hainan University,Sanya,Hainan 572025,China;School of Tropical Agriculture and Forestry/SARA/SRR,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学南繁学院/三亚南繁研究院,海南三亚572025 [2]海南大学热带农林学院/农业农村学院/乡村振兴学院,海口570228

出　　处：《热带生物学报》2024年第6期718-727,共10页Journal of Tropical Biology

基　　金：海南省自然科学基金(321RC465)资助。

摘　　要：超氧化物歧化酶(SOD)是植物抗氧化系统中的一类重要酶,在植物生长发育中起着重要作用,为探究杧果SOD(MiSOD)的基因功能,在不同组织与增强UV-B照射下的表达模式。本研究基于杧果基因组,利用生物信息学方法,从蛋白质特性、系统进化关系、基因结构、启动子顺式作用元件等多个方面鉴定MiSOD基因家族成员,并通过转录组分析鉴定MiSOD基因家族成员的表达模式。杧果中共鉴定得到10个SOD基因,MiSOD蛋白大多属于稳定蛋白与酸性蛋白。多物种SOD蛋白系统发育分析表明,SOD按照蛋白类型被聚类为三个亚组。基因结构分析显示,SOD基因含有5~8个内含子,同一进化分支的基因,结构更为相似,其蛋白的保守基序与保守结构域也相同。10个MiSOD分布于7条染色体与1条碎片片段上,包含3对片段复制基因。顺式作用元件分析表明,MiSOD成员启动子中包含生长发育元件、激素响应元件和逆境响应相关元件。MiSOD基因在“Alphonso”杧果不同组织中表达模式不同,MiCu/Zn-SOD3、MiFe-SOD1、2、3在不同组织中表达量均较低,果肉、叶片、树皮和果皮中MiCSD4表达量最高,种子中MiMSD1表达量最高,根与花中,MiCSD1表达量最高;在增强UV-B照射的“台农一号”杧果果肉中,与对照相比,MiSOD基因没有显著差异。综上所述,MiSOD基因家族成员可能通过感受不同种类的信号而行使不同的功能,从而形成不同的表达模式;在“台农一号”杧果响应UV-B胁迫中,SOD可能不起关键作用。本研究为进一步研究MiSOD基因对不同信号的响应机制奠定基础。Superoxide dismutase(SOD)is an important enzyme in the plant antioxidant system,playing a crucial role in plant growth and development.An attempt was made to analyze the gene function of MiSOD and its expression patterns in different tissues and under enhanced UV-B irradiation.Members of the MiSOD gene family from multiple aspects such as protein characteristics,phylogenetic relationships,gene structure,and promoter cis acting elements were identified by using the mango genome and bioinformatics methods,and the expression patterns of MiSOD gene family members were identified by using the transcriptome analysis.A total of 10 SOD genes were identified in the mango fruit,and most of the MiSOD proteins belong to stable proteins and acidic proteins.The phylogenetic analysis of multiple species SOD proteins showed that SOD was clustered into three subgroups according to protein types.Gene structure analysis shows that the SOD gene contains 5-8 introns,and genes from the same evolutionary branch have more similar structures.The conserved motifs and domains of their proteins are also the same.Ten MiSOD are distributed on 7 chromosomes and 1 fragment,containing 3 segmental duplication genes.The analysis of cis acting elements showed that the promoters of MiSOD members contain growth and development elements,hormone response elements,and stress response related elements.The expression pattern of MiSOD genes varies in different tissues of‘Alphonso’mango.MiCSD3,MiFSD1,2,3 have low expression levels in different tissues;the expression level of MiCSD4 is highest in the flesh,leaves,bark,and peel;the expression level of MiMSD1 is highest in the seeds,and the expression level of MiCSD1 is highest in the roots and flowers.Compared with the control,there was no significant difference in the MiSOD genes in the flesh of“Tainong-1”mango under enhanced UV-B irradiation.In summary,members of the MiSOD gene family may exert different functions by sensing different types of signals,thereby forming different expression patterns.SO

关 键 词：杧果 超氧化物歧化酶(SOD) 基因家族 表达模式 

分 类 号：S667.7[农业科学—果树学]