西沙软珊瑚Sarcophyton crassocaule中具有抗炎活性的西松烷二萜  

Cembranoids with anti-inflammatory activity extracted from the soft coral Sarcophyton crassocaule collected off the Xisha Islands

作　　者：丘穗萍 畅文军[2] 王昊[2] 戴好富[2] 曾艳波[1,2] QIU Suiping;CHANG Wenjun;WANG Hao;DAI Haofu;ZENG Yanbo(College of Marine Biology and Fisheries,Hainan University,Haikou,Hainan 570228;Hainan Key Laboratory for Research and Utilization of Functional Components of Marine Bioresources,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China)

机构地区：[1]海南大学海洋生物与水产学院,海口570228 [2]中国热带农业科学院热带生物技术研究所海南省海洋生物资源功能性成分研究与利用重点实验室,海口571101

出　　处：《热带生物学报》2024年第6期812-822,共11页Journal of Tropical Biology

基　　金：海南省重点研发计划项目(ZDYF2021SHFZ107);国家自然科学基金项目(41776093);中国热带农业科学院国家热带农业科学中心科技创新团队项目(CATASCXTD202416)。

摘　　要：采集来自中国南海西沙群岛的肥厚肉芝软珊瑚(Sarcophyton crassocaule),研究其化学成分及生物活性。采用硅胶柱层析、薄层层析、凝胶(Sephadex-20)柱层析和半制备高效液相(semi-preparative highperformance liquid chromatography,Semi-Prep HPLC)等分离方法,对S.crassocaule的乙酸乙酯相进行分离纯化,得到11个化合物。将其理化特性和波谱数据与文献数据进行比对,鉴定这11个化合物的结构分别为:sarsolilide A(1)、trochelioid A(2)、(1R^(*),2R^(*),7R^(*),8R^(*),15R^(*),3E,11E)-7,8:1,15-diepoxycembra-3,11-dien-16,2-olide(3)、(+)-7α,8β-dihydroxyde-epoxysarcophine(4)、1,15β-epoxy-deoxy-sarcophine(5)、(-)-isosarcophine(6)、cherbonolide A(7)、sarcomililatin A(8)、trocheliolide A(9)、lobophytin B(10)和(+)-sarcophine(11)。化合物1~11均为二萜类化合物,其中化合物3首次在S.crassocaule中被分离到。抗炎活性测试结果表明,sarcomililatin A(8)能够抑制脂多糖(LPS)诱导的RAW264.7细胞中NO的生成,其IC50值为(35.60±3.10)μmol·L^(-1),其他化合物没有明显的抗炎活性。An attempt was made to identify chemical constituents and their biological activities of Sarcophyton crassocaule,a soft coral,collected off the Xisha Islands in the South China Sea.Using silica gel column chromatography,thin-layer chromatography,Sephadex-20 gel column chromatography,and semi-preparative highperformance liquid chromatography(Semi-Prep HPLC),the ethyl acetate fraction of S.crassocaule was separated and purified,from which 11 compounds were yielded.The physical and chemical properties and spectroscopic data of these compounds were compared with those in the literature.The 11 compounds were identified assarsolilide A(1);trochelioid A(2);(1R^(*),2R^(*),7R^(*),8R^(*),15R^(*),3E,11E)-7,8:1,15-diepoxycembra-3,11-dien-16,2-olide(3);(+)-7α,8β-dihydroxy-deepoxy-sarcophine(4);1,15β-epoxy-deoxysarcophine(5);(-)-isosarcophine(6);cherbonolide A(7);sarcomililatin A(8);trocheliolide A(9);lobophytin B(10);(+)-sarcophine(11).Compounds 1~11 are diterpenoids.Compound 3 was identified in S.crassocaule for the first time.The anti-inflammatory activity test showed that sarcomililatin A(8)could inhibit the production of NO in RAW264.7 cells induced by LPS,with the IC50 value of(35.60±3.10)μmol·L-1,while the other compounds did not exhibit significant anti-inflammatory activity.

关 键 词：软珊瑚 Sarcophyton crassocaule 化学成分 二萜 抗炎活性 

分 类 号：P734.5[天文地球—海洋化学]