添食枯草芽孢杆菌制剂对柞蚕消化吸收的影响  

Effects of Feeding Bacillus subtilis Agent on Digestion and Absorption of Antheraea pernyi

作　　者：全爽 韩冰 郭天凯[1] 杜占军[1] Quan Shuang;Han Bing;Guo Tiankai;Du Zhanjun(Liaoning Institute of Sericultural Science,Fengcheng Liaoning 118100,China)

机构地区：[1]辽宁省蚕业科学研究所,辽宁凤城118100

出　　处：《蚕业科学》2024年第4期378-383,共6页ACTA SERICOLOGICA SINICA

基　　金：财政部和农业农村部国家现代农业产业技术体系项目,农业农村部辽宁野蚕科学观测实验站项目(23-S-15);国家桑蚕改良中心辽宁柞蚕分中心项目(2015-A2211-210682-A0105-002);辽宁省野蚕研究重点实验室项目(2003)。

摘　　要：为提高柞蚕饲料利用效率,以饲用益生菌枯草芽孢杆菌制剂为添食材料,探究其对柞蚕幼虫消化吸收率、中肠围食膜形态结构及消化酶活性的影响。结果显示,添食枯草芽孢杆菌制剂提高了柞蚕4龄和5龄幼虫的消化吸收率,添食组柞蚕5龄幼虫中肠围食膜结构紧凑,贴近中肠上皮细胞。同时,添食枯草芽孢杆菌制剂能提高肠道中相关消化酶的活性,促进柞蚕饲料的消化吸收。因此,通过添食枯草芽孢杆菌制剂能够改变柞蚕幼虫中肠围食膜结构形态,增强相关消化酶活性,进而提高消化吸收率,具有良好的应用前景。In order to improve the feed resource utilization efficiency of Antheraea pernyi,the probiotic Bacillus subtilis agent was used as a feeding material to investigate its effects on the digestibility of Antheraea pernyi larvae,the morpho-logy and structure of midgut peritrophic membrane,and the activity of digestive enzyme.The results showed that feeding Bacillus subtilis agent increased the digestibility of the 4th and 5th instar larvae.The midgut peritrophic membrane of the 5th instar larvae in feeding group was compact and close to midgut epithelial cells.The addition of Bacillus subtilis agent could increase the activity of digestive enzymes in the intestine and promote the digestion and absorption of tussah feed.Therefore,feeding Bacillus subtilis agent can change the morphology of the midgut peritrophic membrane of Antheraea pernyi larvae,increase the activity of related digestive enzymes and thus improve the digestibility,which has a good prospect of application.

关 键 词：柞蚕 枯草芽孢杆菌 消化吸收率 消化酶 

分 类 号：S881.2[农业科学—特种经济动物饲养] S885.1[农业科学—畜牧兽医]