代谢组学探究牛至精油对绵羊直肠粪便代谢物的影响  

Effects of oregano essential oil on faecalmetabonomics in fattening sheepassessed using LC−MS/MS technology

作　　者：宋淑珍[1] 刘立山 周瑞 SONG Shuzhen;LIU Lishan;ZHOU Rui(Animal&Pasture Science and Green Agricultural Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070,Gansu,China;Life Science and Engineering College,Northwest Minzu University,Lanzhou 730030,Gansu,China)

机构地区：[1]甘肃省农业科学院畜草与绿色农业研究所,甘肃兰州730070 [2]西北民族大学生命与工程学院,甘肃兰州730030

出　　处：《草业科学》2024年第11期2715-2724,共10页Pratacultural Science

基　　金：甘肃省农业科学院农业科技创新专项项目(2021GAAS15);西北民族大学人才引进项目(xbmuyjrc2020018)。

摘　　要：选择体重接近[(20.00±1.27)kg]的萨福克与小尾寒羊杂交公羔12只,随机分为两组,每组6只羊,分别为对照组和牛至精油组。对照组饲喂基础饲粮,牛至精油组饲喂基础饲粮+7 g·(d·sheep)^(-1)牛至精油。预试期10 d,正式期72 d,试验期结束后,采集直肠粪便进行液相色谱-串联质谱法(LC-MS/MS)非靶向代谢组学技术筛选差异代谢物。结果表明:1)两组试验羊的粪便中正、负离子模式共筛选出甲基-2,3-O-二烯丙基-4,6-O-亚苄基-alpha-D-吡喃甘露糖苷、二十二碳-4,7,10,13,16-五烯酸酯、甲基胍、腺嘌呤、18:1碳链溶血磷脂酰乙醇胺、N-乙酰-D-神经氨酸、环己烷羧酸、4-甲氧基苯乙酸、DL-多巴、胸腺嘧啶、去甲氧基姜黄素、5′-甲基硫代腺苷、间羟基苯甲酸、S-脱落酸、8-羟基鸟苷15种差异代谢物(|log2FC|>1,P<0.01,变量投影重要度>1),且与对照组相比,牛至精油组的差异代谢物全部上调。2)差异代谢物KEGG通路富集到萜类和类固醇的合成,微生物代谢,苯丙氨酸、酪氨酸和色氨酸的合成,半胱氨酸和蛋氨酸代谢,芳香族化合物的降解,代谢途径共6条通路(拓扑影响值>0.1),分别归属于脂质代谢、有机物分解与合成代谢和氨基酸代谢。这表明,牛至精油可能参与育肥羊脂质代谢、有机物代谢和氨基酸代谢,通过调节代谢途径发挥其在羊养殖中的健康维护作用。Twelve Suffolk Ram×Small-tailed Han sheep with similar body weight[(20.00±1.27)kg]were randomly assigned to 2 treatments(control,oregano essential oil group),6 sheep per group.The control group was fed a basic diet,the oregano essential oil group was fed a basic diet+7 g·d^(-1) of oregano essential oil,to each sheep,separately.A pre-trial period of 10 d and formal period was 72 d was employed.The feces of the control and oregano essential oil groups were collected at the end of the trialfor screening of differential metabolites by liquid chromatography-tandem mass spectrometry(LC-MS/MS)technology.The results identified multiple differential metabolites,including:1)Heliangin,CE[22:5(4Z,7Z,10Z,13Z,16Z)],methylguanidine,acadesine,LysoPE[18:1(9Z)/0:0],N-Acetyl-D-neuraminic acid,cyclohexanecarboxylic acid,4-methoxyphenylacetic acid,DL-dopa,thymidine,demethoxycurcumine,5′-methylthioadenosine,3-hydroxybenzoic acid,(S)-abscisic acid,8-hydroxyguanosin.A total of 15 differential metabolites were screened by positive and negative ion patternsin the feces of the two groups(|log2FC|>1,P<0.01,VIP>1).Compared with the control group,all metabolites were up-regulated in the oregano essential oil group.2)The differential metabolite KEGG pathway analyses demonstrated that biosynthesis of terpenoids and steroids,microbial metabolism in diverse environments,phenylalanine,tyrosine and tryptophan biosynthesis,cysteine and methionine metabolism,degradation of aromatic compounds and metabolic pathways were enriched in the oregano essential oil group(Impact>0.1).These pathways belonged to lipid metabolism,organic matter metabolism and amino acid metabolism,separately.In conclusion,oregano essential oil may take part in metabolism of lipids,organics and amino acids in fattening sheep,which plays the important role in maintaining health in sheep by regulating metabolic pathway.

关 键 词：牛至精油 羊 代谢物 芳香族化合物 脂质代谢 有机物代谢 氨基酸代谢 

分 类 号：S826.5[农业科学—畜牧学]