不同粗饲料来源对湖羊羔羊盲肠菌群结构及功能的影响  

Effects of different dietary forage sources on the structure and function of cecum bacteria in Hu lambs

作　　者：任春燕[1] 赵海碧 邴睿 刘婷[1] REN Chunyan;ZHAO Haibi;BING Rui;LIU Ting(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070

出　　处：《草业科学》2024年第11期2725-2734,共10页Pratacultural Science

基　　金：公招博士科研启动金项目(GAU-KYQD-2018-28);国家自然科学基金项目(32160800、31860656)。

摘　　要：选用7日龄[(3.77±0.46)kg]湖羊公羔60只,随机分为4个处理组,饲草来源组饲喂苜蓿(Medicago sativa)干草(alfalfa hay,AH)和燕麦(Avenasativa)干草(oat hay,OH),非饲草来源组饲喂甜菜(Betavulgaris)粕(beet pulp,BP)和大豆(Glycine max)皮(soybean hulls,SH)。70日龄时饲养试验结束,各组随机屠宰6只羔羊,获得24个盲肠内容物样品以备16S rDNA测序,研究饲粮粗饲料来源对湖羊羔羊盲肠内容物菌群结构和功能的影响。结果表明:24个盲肠内容物样品共获得72482条有效数据与2999个OTU,物种注释共获得12个门、54个纲、103个目、174个科和349个属;OH组物种数、辛普森指数和香农指数显著高于AH组(P<0.05);盲肠以厚壁菌门和拟杆菌门为核心菌门;瘤胃球菌科为优势菌科;OH组毛螺旋菌科显著高于AH和SH组(P<0.05),克里斯滕森菌科显著高于AH组和BP组(P<0.05);BP组变形菌门显著高于AH、SH和OH组(P<0.05),毛螺旋菌科极显著高于AH和SH组(P<0.01);SH和OH组未鉴定的丹毒丝菌属显著高于AH和BP组。使用Tax4Fun法的预测分析得出湖羔羊盲肠菌群以碳水化合物代谢、脂类、能量和氨基酸代谢为主。相关性分析Muribaculaceae菌科与活体重和日增重之间呈显著正相关关系(P<0.05),普雷沃氏菌科与活体重和日增重之间呈显著负相关关系(P<0.05)。综上所述,开食料不同粗饲料来源由于其纤维素结构不同,对湖羊羔羊盲肠微生物菌群结构及功能作用具有较大差异。The purpose of this experiment was to investigate the effect of forage sources on the structure and function of cecum bacteria in Hu lambs.Sixty seven-day-old[(3.77±0.46)kg]Hu lambs were divided into four groups of 15 lambs in each group.The forage sources were alfalfa(Medicago sativa)hay(AH)and oat(Avena sativa)hay(OH),whereas the non-forage sources were beet pulp(Beta vulgaris)(BP)and soybean hulls(Glycine max)(SH).The lambs were allowed to freely feed and drink throughout the entire process.At the age of 70 days,six lambs were randomly selected from each of the treatment group and slaughtered.Twenty-four samples of cecum contents were collected for 16S rDNA sequencing.The results showed that 72482 sequence data and 2999 operational taxonomic units(OTU)were obtained from 24 cecal content samples,and 12 phyla,54 classes,103 orders,174 families,and 349 genera were obtained from species annotations.The observed species index,Simpson index,and Shannon index in OH group were significantly higher than those in AH group(P<0.05).Firmicutes and Bacteroidetes were the most abundant phyla in the cecum,and Rumenococcus was the dominant family.Trichospiraceae in OH group was significantly higher than that in AH and SH groups,and Christensenaceae in OH group was significantly higher than that in AH and BP groups(P<0.05).Proteobacteria in BP group was significantly higher than that in AH,SH,and OH groups(P<0.05),and Spirochaetes in BP group was significantly higher than that in AH and SH groups(P<0.01).Anaerostipes were significantly higher in the OH group than in the AH group(P<0.05),and unidentified Erysipelotrichaceae spp.were significantly higher in the SH and OH groups than in the AH and BP groups(P<0.05).Functional prediction analysis using the Tax4Fun method revealed that carbohydrate,lipid,energy,and amino acid metabolisms were the predominant metabolisms in the cecum microbiota of lake lambs.Correlation analysis showed a positive correlation between Muribaculaceae and live weight and daily weight gain(P<0.05),and a

关 键 词：湖羊 粗饲料来源 盲肠 微生物结构 微生物功能 羔羊 16S rDNA 

分 类 号：S826.5[农业科学—畜牧学]