Optimized pretreatment increases the susceptibility of hepatitis B virus infection by enhancing actomyosin-driven cell spreading  

作　　者：Shuzhi Cui Wei Gao Zonghong Li Yi Xu Yaming Jiu 

机构地区：[1]Guangzhou Institute of Pediatrics,Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangdong,China [2]Unit of Cell Biology and Imaging Study of Pathogen Host Interaction,Key Laboratory of Molecular Virology and Immunology,Shanghai Institute of Immunity and Infection,Chinese Academy of Sciences,Shanghai,China [3]University of Chinese Academy of Sciences,Beijing,China [4]Guangzhou National Laboratory,Guangdong,China

出　　处：《hLife》2024年第4期201-205,共5页健康科学（英文）

基　　金：supported by the National Natural Science Foundation of China(32222022,92354301,92054104);Key Research and Development Program,Ministry of Science and Technology of China(2022YFC2303502,2021YFC2300204);Natural Science Foundation of Shanghai(23ZR1470900).

摘　　要：Hepatitis B virus(HBV),an enveloped DNA virus with a partially double-stranded DNA genome,causes transient and chronic hepatitis B in humans.A certain amount of chronic HBV infections eventually leads to liver cirrhosis or hepatocellular carcinoma which is a major health problem worldwide[1,2].With tissue tropism and species-specific infection,the establishment of in vitro models permissive to HBV is critical in understanding the viral life cycle and designing curative therapies.The ideal cell model for in vitro infection studies is primary human hepatocytes(PHHs)[3].However,PHHis difficult to obtain andwith high cost.The donor diversity and high batch-to-batch variability of infection efficiency also limit its applicability.In 2012,sodium taurocholate co-transporting polypeptide(NTCP)was identified as a functional receptor for HBV entry[4].Thus,a new infection model based on NTCP-reconstituted HepG2 hepatocytes(HepG2-NTCP)was successfully established,which greatly promoted the understanding of HBV infection and replication[4].However,in this infection system,successful HBV infection stringently requires a high concentration of HBV viral particles,but still in most cases supports a low level of HBV replication[5].Thus,further optimization of infection cell models is an important demand in HBV research.

关 键 词：INFECTION BATCH eventually 

分 类 号：R512.62[医药卫生—内科学]