Brainstem opioid peptidergic neurons regulate cough reflexes in mice  

作　　者：Haicheng Lu Guoqing Chen Miao Zhao Huating Gu Wenxuan Zheng Xiating Li Meizhu Huang Dandan Geng Minhui Yu Xuyan Guan Li Zhang Huimeng Song Yaning Li Menghua Wu Fan Zhang Dapeng Li Qingfeng Wu Congping Shang Zhiyong Xie Peng Cao 

机构地区：[1]National Institute of Biological Sciences,Beijing 102206,China [2]Tsinghua Institute of Multidisciplinary Biomedical Research,Tsinghua University,Beijing 100084,China [3]Peking University-Tsinghua University-NIBS Joint Graduate Program,School of Life Sciences,Tsinghua University,Beijing 100084,China [4]Department of Neurobiology,School of Basic Medical Sciences,Beijing Key Laboratory of Neural Regeneration and Repair,Advanced Innovation Center for Human Brain Protection,Capital Medical University,Beijing 100069,China [5]Key Laboratory of Neural and Vascular Biology,Ministry of Education,Department of Biochemistry and Molecular Biology,Hebei Medical University,Shijiazhuang 050011,China [6]Graduate School of Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100005,China [7]State Key Laboratory of Molecular Development Biology,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China [8]School of Basic Medical Sciences,Guangzhou National Laboratory,Fifth Affiliated Hospital,Guangzhou Medical University,Guangzhou 510799,China [9]Department of Psychological Medicine,Zhongshan Hospital,Institute for Translational Brain Research,State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200433,China

出　　处：《The Innovation》2024年第6期50-61,共12页创新（英文）

基　　金：supported by the National Natural Science Foundation of China(31925019 to P.C.);the STI 2030 Major Projects(2021ZD0202701to P.C.);the New Cornerstone Science Foundation through the XPLORER PRIZE(to P.C.).All data are archived in NIBS.

摘　　要：Cough is a vital defensive reflex for expelling harmful substances from the airway.The sensory afferents for the cough reflex have been intensively studied.However,the brain mechanisms underlying the cough reflex remain poorly understood.Here,we developed a paradigm to quantitatively measure cough-like reflexes in mice.Using this paradigm,we found that prodynorphin-expressing(Pdyn+)neurons in the nucleus of the solitary tract(NTS)are critical for capsaicin-induced cough-like reflexes.These neurons receive cough-related neural signals from Trpv1+vagal sensory neurons.The activation of Pdyn+NTS neurons triggered respiratory responses resembling cough-like reflexes.Among the divergent projections of Pdyn+NTS neurons,a glutamatergic pathway projecting to the caudal ventral respiratory group(cVRG),the canonical cough center,was necessary and sufficient for capsaicin-induced cough-like reflexes.These results reveal that Pdyn+NTS neurons,as a key neuronal population at the entry point of the vagus nerve to the brainstem,initiate cough-like reflexes in mice.

关 键 词：RESPIRATORY OPIOID COUGH 

分 类 号：R97[医药卫生—药品]