昆明山海棠根茎的化学成分及其抗肿瘤活性研究  

Study on chemical constituents and anti-tumor activity from the root of Tripterygium hypoglaucum

作　　者：王计宏 杨碧娟 孙玉梅 黄杰林 张殿昊 尹俊林 曾广智 WANG Ji-hong;YANG Bi-juan;SUN Yu-mei;HUANG Jie-lin;ZHANG Dian-hao;YIN Jun-lin;ZENG Guang-zhi(Key Laboratory of Chemistry in Ethnic Medicinal Resource,State Ethnic Affairs Commission&Minstry of Education,Yunnan Minzu University,Kunming 650500,China;School of Ethnic Medicine,Yunnan Minzu University,Kunming 650500,China)

机构地区：[1]云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明650500 [2]云南民族大学民族医药学院,云南昆明650500

出　　处：《云南民族大学学报（自然科学版）》2024年第6期671-679,共9页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(31760095,81960639,21768005)。

摘　　要：为研究昆明山海棠(Tripterygium hypoglaucum)根茎的化学成分,运用重结晶、硅胶柱色谱、半制备型高效液相、制备型中压液相、葡聚糖凝胶Sephadex LH-20柱色谱等分离纯化手段,对昆明山海棠根茎95%甲醇提取物进行化学成分研究,共分离得到20个化合物.通过现代波谱解析等方法确定了化合物结构为:3-羟基-2-O-3-无羁萜烯-29-羧酸(1)、3-epi-triptobenzene B(2)、角鲨烯(3)、β-谷甾醇(4)、(6R,E)-6-((3S,8S,9S,10R,13R,14S,17R)-10,13-dimethyl-3-(palmitoyloxy)-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl)-3-ethyl-2-methylhept-4-enoic acid(5)、无羁萜(6)、麦珠子酚(7)、3,7-二甲基-正辛基3α醇-1-苯甲酸酯(8)、16α-羟基-对映-贝壳杉烷-19-酸(9)、triptotin C(10)、雷酚萜醇(11)、雷公藤酸C(12)、4-羟基苯乙醇(13)、香草醇(14)、2-甲氧基-1,4-苯二甲醇(15)、雷公藤内酯(16)、antriptolactone(17)、cangoronine(18)、3-oxo-29-hydroxyfiedelane(19)、3β-hydroxy-D:B-friedoolean-5-en-29-oic acid(20).化合物1、5、7、8、9、13、14和15首次从该属植物中分离,化合物2、10、19和20首次从昆明山海棠中分离.活性研究发现在100μmol/L浓度下,化合物1、2、5具有一定的细胞毒性,其中化合物2对肿瘤细胞株HepG2、BCAP-37、B16细胞均具有中等细胞毒性,其IC_(50)值分别为(35.79±1.0)、(32.03±1.1)和(37.44±1.5)μmol/L.To study the chemical composition from the root of Tripterygium hypoglaucum,20 compounds were isolated from the 90%methanol extract of Tripterygium hypoglaucum by recrystallization,silica gel column chromatography,semi-preparative highperformance liquid chromatography,preparative medium pressure liquid chromatography and dextran gel Sephadex LH-20 column chromatography.Based on their physical and chemical properties,their structures were identified as 3-hydroxy-2-O-3-friedelen-29-oic acid(1),3-epi-triptobenzene B(2),squalene(3),β-sitosterol(4),(6R,E)-6-((3S,8S,9S,10R,13R,14S,17R)-10,13-dimethyl-3-(palmito-yloxy)-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl)-3-ethyl-2-methylhept-4-enoic acid(5),friedelin(6),alphitol(7),3,7-dimethyl-n-octan-3α-ol-1-yl-benzoate(8),16α-hydroxy-ent-kauran-19-oic acid(9),triptotin C(10),triptonediol(11),wilforic acid C(12),2-(4-hydroxy-phenyl)-ethanol(13),homovanillyl alcohol(14),(2-methoxy-1,4-phenylene)dimethanol(15),triptophenolide(16),antriptolactone(17),cangoronine(18),3-oxo-29-hydroxyfiedelane(19),3β-hydroxy-D:B-friedoolean-5-en-29-oic acid(20).Compounds 1,5,7,8,9,13,14 and 15 were isolated from this genus Tripterygium for the first time,compounds 2,10,19 and 20 were isolated from this Tripterygium hypoglaucum for the first time.The activity study showed that only compounds 1,2 and 5 had certain cytotoxicity at concentration of 100μmol/L,compound 2 had moderate cytotoxicity activity against HepG2,BCAP-37 and B16 cells,the IC_(50) values were(35.79±1.0),(32.03±1.1)and(37.44±1.5)μmol/L,respectively.

关 键 词：昆明山海棠 化学成分 萜类化合物 抗肿瘤细胞株活性 

分 类 号：R932[医药卫生—生药学]