雪上一枝蒿总碱及其主要二萜生物碱的镇痛活性研究  

Study on the analgesic effects of total alkaloid and its main diterpenoid alkaloids isolated from the Aconitum brachypodum Diels

作　　者：苏东雪 彭海燕 庄馨瑛 何芳雁 周志宏 马晓霞 SU Dong-xue;PENG Hai-yan;ZHUANG Xin-ying;HE Fang-yan;ZHOU Zhi-hong;MA Xiao-xia(School of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500

出　　处：《云南民族大学学报（自然科学版）》2024年第6期680-684,共5页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：云南省科技计划项目(2019ZF003);昆明市科技计划项目(2019-1-N-25318000002197)。

摘　　要：分别采用醋酸扭体法和热板法研究雪上一枝蒿总碱及5个主要二萜生物碱成分乌头碱、3-去氧乌头碱、尼奥灵、12-表-15-乙酰欧乌头碱、12-表-欧乌头碱外周和中枢的镇痛活性.与空白对照组比较,雪上一枝蒿总碱高、中剂量组,乌头碱高、中、低剂量组,尼奥灵高、中剂量组,12-表-15-乙酰欧乌头碱的高剂量组和12-表-欧乌头碱的中剂量组对冰醋酸所致的小鼠扭体均具有抑制作用,差异具有统计学意义(P<0.05或P<0.01);与本组基础痛阈值比较,雪上一枝蒿总碱高、中、低剂量组,乌头碱高、中、低剂量组,3-去氧乌头碱高剂量组,尼奥灵、12-表-15-乙酰欧乌头碱以及12-表-欧乌头碱的高、中剂量组在给药15 min后均能显著延长热板所致小鼠疼痛的痛阈值(P<0.05或P<0.01).由此可得,雪上一枝蒿总碱及5个主要二萜生物碱成分均有明显的镇痛作用.To study the analgesic effects of total alkaloid of Aconitum brachypodum and its five main diterpenoid alkaloids,aconitine,3-deoxyaconitine,neoline,12-epi-15-acetylaconitine and 12-epi-aconitine.The peripheral and central analgesic effects were observed by acetic acid writhing method and hot plate method.Compared with the blank control group,the high and middle-dose groups of total alkaloids from Aconitum brachypodum,the high,middle,and low-monitoring groups of aconitine,the high and medium-dose groups of neoline,the high-dose group of 12-epi-15-acetylaconitine and the medium-dose group of 12-epi-aconitine showed significant inhibitory effects on acetic acid-induced writhing in mice(P<0.05 or P<0.01).Compared with the basic pain threshold of this group,the high,middle and low-dose groups of total alkaloids from Aconitum brachypodum,the high,middle,and low-monitoring groups of aconitine,the high-dose group of 3-deoxyaconitine,the high and medium-dose groups of neoline,12-epi-15-acetylaconitine and 12-epi-aconitine could significantly prolong the pain threshold 15 minutes after administration in hot plate mice model(P<0.05 or P<0.01).All of the content of the total alkaloids and five components have obvious analgesic effects.

关 键 词：雪上一枝蒿总碱 二萜生物碱 镇痛 

分 类 号：R284.1[医药卫生—中药学]