Impaired 2′,3′-cyclic phosphate tRNA repair causes thermo-sensitive genic male sterility in rice  

作　　者：Bin Yan Chunyan Liu Jing Sun Yang Mao Can Zhou Ji Li Wei Liu Shengdong Li Wei Yan Chenjian Fu Peng Qin Xingxue Fu Xinghui Zhao Xianwei Song Jiawei Nie Feng Gao Yuanzhu Yang Yuhang Chen Xiaofeng Cao 

机构地区：[1]Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,China [2]Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology,Institute of Biotechnology,Beijing Academy of Agriculture and Forestry Sciences,Beijing,China [3]Laboratory of Molecular Developmental Biology,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,China [4]University of the Chinese Academy of Sciences,Beijing,China [5]Institute of Plant and Food Science,Department of Biology,Southern University of Science and Technology,Shenzhen,Guangdong,China [6]Yuan Longping High-tech Agriculture Co.,Ltd.,Changsha,Hunan,China [7]Key Laboratory of Seed Innovation,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,China [8]State Key Laboratory of Hybrid Rice,Changsha,Hunan,China [9]Key Laboratory of Rice Germplasm Enhancement in Southern China,Ministry of Agriculture and Rural Affairs,Changsha,Hunan,China

出　　处：《Cell Research》2024年第11期763-775,共13页细胞研究（英文版）

基　　金：supported by grants from the Biological Breeding-National Science and Technology Major Project of China(2023ZD04073);the National Key R&D Program of China(2020YFA0509900);the National Natural Science Foundation of China(32330019,32171284,32170620 and 32370633);the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24010302).

摘　　要：Hybrid rice,widely planted in Asia,is pathogen resistant and has superior yields,making it a major contributor to global food security.The two-line hybrid rice system,which utilizes mutants exhibiting photo-/thermo-sensitive genic male sterility(P/TGMS),is the leading hybrid rice breeding technology.Mutations in THERMO-SENSITIVE GENIC MALE STERILE 5(TMS5)accounts for over 95%of current TGMS lines.We previously found that tms5 carries a mutation in ribonuclease ZS1.Despite its importance for breeding robust rice lines,the mechanism underlying tms5-mediated TGMS remains elusive.Here,we demonstrate that TMS5 is a tRNA 2′,3′-cyclic phosphatase.The tms5 mutation leads to accumulation of 2′,3′-cyclic phosphate(cP)-ΔCCA-tRNAs(tRNAs without 3′CCA ended with cP),which is exacerbated by high temperatures,and reduction in the abundance of mature tRNAs,particularly alanine tRNAs(tRNA-Alas).Overexpression of tRNA-Alas in the tms5 mutant restores male fertility to 70%.Remarkably,male fertility of tms5 mutant is completely restored at high temperatures by knocking out OsVms1 which encodes the enzyme for cP-ΔCCA-tRNA generation.Our study reveals the mechanism underlying tms5-mediated TGMS in rice and provides mechanistic insight into the further improvement of TGMS in hybrid crop development.

关 键 词：BREEDING FERTILITY planted 

分 类 号：S511[农业科学—作物学]