基于代谢组学探讨“醒神解郁”法电针对抑郁症患者血清代谢物的影响  

Effect of“Xingshen-Jieyu”electroacupuncture on serum differential metabolites in patients with depression based on metabolomics

作　　者：敖与天 董凯强 王文博 李泓瑶 李佳秀 郭蓉娟[2] AO Yu-tian;DONG Kai-qiang;WANG Wen-bo;LI Hong-yao;LI Jia-xiu;GUO Rong-juan(Beijing University of Chinese Medicine,Beijing 100029,China;Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078)

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东方医院,北京100078

出　　处：《针刺研究》2024年第11期1181-1189,共9页Acupuncture Research

基　　金：国家自然科学基金联合基金项目(No.U21A20401)。

摘　　要：目的:通过血清代谢组学技术,观察“醒神解郁”法电针对抑郁症患者血清代谢物的影响,探讨其效应机制。方法:抑郁症患者15例纳入电针组,并匹配健康受试者15例入健康组。电针组采用“醒神解郁”法电针治疗,每次30 min,每周3次,连续6周。治疗前后,采用汉密尔顿抑郁量表17项(HAMD-17)和汉密尔顿焦虑量表(HAMA)评估患者焦虑抑郁程度;采用匹兹堡睡眠质量指数量表(PSQI)评估患者睡眠情况;采用疲劳量表14项(FS-14)评价患者的疲劳程度;采集健康受试者及抑郁症患者血清进行代谢组学检测,筛选差异代谢物,并进行通路富集分析。结果:与治疗前比较,抑郁症患者治疗后HAMD-17、HAMA、PSQI、FS-14评分下降(P<0.05)。抑郁症患者与健康受试者比较,筛选出46种差异代谢物;“醒神解郁”法电针治疗前后对比,筛选出19种差异代谢物,其中4种在抑郁症患者中异常下调的代谢物在电针治疗后上调。对电针治疗前后差异代谢物进行KEGG分析,共富集到19种代谢通路,主要涉及谷氨酸能突触、GABA能突触及多条氨基酸代谢相关通路。结论:“醒神解郁”法电针可能通过调节谷氨酸与GABA,起到改善抑郁症的作用。Objective To explore the effect of“Xingshen-Jieyu”(inducing resuscitation and dispel ling melancholy)electroacupuncture(EA)on serum metabolites in patients with depression and its underlying mechanisms through serum non-targeted metabolomics.Methods Fifteen depression patients were subjected into the EA group,and 15 healthy volunteers were matched into the healthy group.EA(2 Hz/15 Hz,a tolerable strength)was applied to Baihui(GV20),Shenting(GV24),Toulinqi(GB15),Benshen(GB13),Touwei(ST8),Xuanli(GB6)and Qubin(GB7)for 30 min,3 times a week for 6 weeks.The severity of depression was assessed using Hamilton Depression Scale-17(HAMD-17)and Hamilton Anxiety Scale(HAMA),the degree of fatigue was evaluated using Fatigue Scale-14(FS-14).The subjects sleep quality was assessed using Pittsburgh sleep quality index(PSQI).Blood samples were collected before and after the treatment for processing serum to perform non-targeted metabolomics profile detection using ultra-high-performance liquid chromatography coupled with mass spectrometry(UPLC-MS),and Partial Least Squares Discriminant Analysis(PLS-DA,a statistical approach).Differential metabolites were screened and pathway enrichment analysis was performed.Results After the treatment,the scores of HAMD-17,HAMA,PSQI and FS-14 of depression patients were significantly lowered in the EA group(P<0.05).Compared with the healthy subjects,46 differential metabolites(21 up-regulated,25 down-regulated)were screened in patients with depression.Compared with pre-treatment in the EA group,19 differential(17 up-regulated,and 2 down-regulated)metabolites were screened.Four down-regulated metabolites of the 46 differential metabolites in depression patients were recovered after EA treatment.Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis of differential metabolites before and after EA showed that 19 metabolic pathways were enriched,mainly involving glutaminergic synapses,GABAergic synapses and several amino acid metabolism related pathways.Conclusion“Xingshen-Jieyu”EA mainly reg

关 键 词：抑郁症 电针 “醒神解郁” 代谢组学 血清 

分 类 号：R246.6[医药卫生—针灸推拿学]