玉米秸秆降解复合菌群的驯化及培养条件优化  

Domestication and optimization of cultivation conditions of the composite Microorganisms for corn stover degradation

作　　者：葛昊罡 李雨佳 蒋博乐 刘依娜 孟庆欣 GE Haogang;LI Yujia;JIANG Bole;LIU Yina;MENG Qingxin(School of Life Sciences,Agriculture and Forestry,Qiqihar University,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《高师理科学刊》2024年第11期54-62,共9页Journal of Science of Teachers＇College and University

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(145309326)。

摘　　要：将具有崩解滤纸能力的复合菌群在60℃培养条件下以玉米秸秆为碳源连续驯化19代,分析17~19代的稳定性,对比原始样本和第19代复合菌群的微生物组成,并通过响应面法改良培养条件.结果表明,17~19代复合菌群滤纸酶活力和秸秆降解率差异性不显著,pH变化趋势相同.经过连续驯化,部分微生物被淘汰,菌群丰度下降.在门水平上,驯化后的优势菌群由厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)和放线菌门(Actinobacteriota)变为厚壁菌门和变形菌门,在属水平上,驯化后的优势菌群由棒杆菌属(Corynebacteriumsp.)和咸海鲜球菌属(Jeotgalicoccus sp.)变为嗜热梭菌属(Thermoclostridium sp.)和尿素芽孢杆菌属(Ureibacillus sp.),表明这些优势菌群对玉米秸秆具有较强的降解潜力.利用响应面法优化的最佳培养条件为:pH6.11,氮源添加量0.68%,秸秆粉添加量0.70%.Microbial consortium possessing the capability to degrade filter paper was subjected to continuous domestication for 19 generations at 60℃ with corn stover as the sole carbon source.The stability of the consortium from generations 17~19 was evaluated,and the microbial compositions of the original sample and the 19th generation consortium were compared.The culture conditions were optimized using response surface methodology.The results demonstrated that there were no significant differences in filter paper enzyme activity and degradation rate of corn stover between generations 17~19,and the variation trends of pH were similar.Continuous domestication led to the elimination of some microorganisms,resulting in the decrease of microbial richness.At the phylum level,the dominant taxa in the domesticated consortium changed from Firmicutes,Proteobacteria and Actinobacteriota to Firmicutes and Proteobacteria.At the genus level,the dominant taxa shifted from Corynebacterium sp.and Jeotgalicoccus sp.to Thermoclostridium sp.and Ureibacillus sp.,suggesting that these taxa have a high potential for corn stover degradation.Response surface methodology was used to optimize cultivation conditions.Optimal cultivation conditions were determined as follows,pH value 6.11,nitrogen source addition 0.68% and corn stover powder addition 0.70%.

关 键 词：复合菌群 驯化 玉米秸秆 优势菌群 培养条件 

分 类 号：Q93[生物学—微生物学]