蜂针直刺治疗急性痛风性关节炎的血清蛋白质组学研究  

Serum Proteomic Study of the Ameliorative Effect of Direct Bee-Acupuncture in Acute Gouty Arthritis

作　　者：黄中梁 老锦雄[1] 李子勇[1] HUANG Zhongliang;LAO Jinxiong;LI Ziyong(Foshan Hospital of Traditional Chinese Medicine,Foshan 528000,Guangdong China)

机构地区：[1]佛山市中医院,广东佛山528000

出　　处：《中国中医骨伤科杂志》2024年第11期55-62,共8页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：佛山市自筹经费类科技创新项目(2220001004656)。

摘　　要：目的:血清蛋白质组学方法研究蜂针直刺改善急性痛风性关节炎的作用。方法:抗体阵列检测急性痛风性关节炎患者与健康志愿者血清中50种蛋白质的表达差异;GO富集分析急性痛风性关节炎患者差异表达蛋白质功能;ELISA检测分析蜂针直刺治疗前后特异性蛋白质表达变化及炎症指标变化;受试者工作特征(ROC)曲线分析四种差异性表达蛋白质诊断急性痛风性关节炎。结果:基于蛋白质组学的研究,已成功鉴别出四种针对急性痛风性关节炎具有特异性的蛋白质,包括TNF-RⅡ、MIP-1β、IL-8和GM-CSF。富集分析结果揭示差异表达的蛋白质与炎症过程、代谢活动及细胞因子通路的调控等密切相关。ELISA检测结果显示蜂针直刺治疗前后急性痛风性关节炎患者血清TNF-RⅡ、MIP-1β、IL-8和GM-CSF水平差异明显,与治疗前比较,治疗后急性痛风性关节炎患者血清TNF-RⅡ、MIP-1β和IL-8水平显著降低(P<0.01),GM-CSF水平显著升高(P<0.01)。ROC曲线分析结果显示IL-8诊断急性痛风性关节炎患者的价值最高,MIP-1β次之。结论:蜂针直刺可能是通过调控GM-CSF、IL-8、MIP-1β和TNF-RⅡ等特异性蛋白质的表达,从而改善急性痛风性关节炎患者炎症状态,提高机体抗炎能力。Objective:Serum proteomic study of the effect of direct bee-acupuncture to improve acute gouty arthritis.Methods:Antibody array detected the expression differences of 50 proteins in the serum of patients with acute gouty arthritis and healthy volunteers;GO enrichment analyzed the function of differentially expressed proteins in patients with acute gouty arthritis;ROC curve analyzed four differentially expressed proteins in the diagnosis of acute gouty arthritis;ELISA analyzed the changes in the expression of specific proteins and inflammatory indexes before and after the treatment of direct bee-acupuncture stimulation.Results:Four proteins specific to acute gouty arthritis were identified based on proteomics,including TNF-RⅡ,MIP-1β,IL-8,and GM-CSF,and enrichment analysis showed that the differentially expressed proteins were associated with inflammation,metabolism,and cytokine pathways,etc.ROC curve analysis showed that IL-8 had the highest value in diagnosing acute gouty arthritis patients,followed by MIP-1β,and ELISA analysis showed that the expression of the four differentially expressed proteins was associated with inflammation,metabolism,and cytokine pathways.The ELISA results showed that there were significant differences in serum TNF-RⅡ,MIP-1β,IL-8 and GM-CSF levels in patients with acute gouty arthritis before and after direct bee-acupuncture stimulation treatment,and compared with the levels before treatment,the levels of serum TNF-RⅡ,MIP-1βand IL-8 in patients with acute gouty arthritis were significantly lower(P<0.01)and the levels of GM-CSF were significantly higher(P<0.01)after treatment.Conclusion:Direct bee-acupuncture stimulation may improve the inflammatory state of patients with acute gouty arthritis and improve the anti-inflammatory ability of the body by regulating the expression of specific proteins such as GM-CSF,IL-8,MIP-1βand TNF-RⅡ.

关 键 词：蜂针直刺 急性痛风性关节炎 血清蛋白质组学 

分 类 号：R255.6[医药卫生—中医内科学]