饲料磷水平对虎龙杂交斑生长、骨矿化及肝脏、血液相关生理生化指标的影响  

Effect of dietary phosphorus levels on growth,bone mineralization and relatively physiological and biochemical indices in blood and liver of hybrid grouper

作　　者：周智愚 邓银生 张金枫 梁程锦 吴小易[1,2] ZHOU Zhiyu;DENG Yinsheng;ZHANG Jinfeng;LIANG Chengjin;WU Xiaoyi(Hainan University/Hainan Provincial Key Laboratory for Tropical Hydrobi ology and Biotechnology,Haikou 570228,China;Hainan University/State Key Laboratory of Marine Resource Utilization in South China Sea,Haikou 570228,China)

机构地区：[1]海南大学/海南省热带水生生物技术研究重点实验室,海南海口570228 [2]海南大学/南海海洋资源利用国家重点实验室,海南海口570228

出　　处：《中山大学学报（自然科学版）（中英文）》2025年第1期147-157,共11页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：海南省重点研发计划项目(ZDYF2022XDNY157)。

摘　　要：本文旨在探究饲料不同磷(P)水平对虎龙杂交斑生长、骨矿化及肝脏、血液相关生理生化指标的影响。实验设计了7组以干物质计,其等能(以每100 g计)、等蛋白、等脂分别为1421.2 kJ、47%、8%的实验饲料,饲料中磷的实测值分别为1.05%、1.10%、1.20%、1.37%、1.52%、1.59%和1.70%,相应缩写为P1.05、P1.10、P1.20、P1.37、P1.52、P1.59及P1.70,每组3个平行。实验鱼[初始体质量(12.14±0.05)g]每日表观饱食投喂两次(8:00和16:30),养殖周期为6周。分析结果显示,P1.05组体质量增长率(WG)、特定生长率(SGR)和肥满度(CF)均极显著高于其他实验组(P<0.01),蛋白质增长值(PPV)在P1.05和P1.10组高于其他实验组,但各组间无显著差异(P>0.05)。随着饲料磷水平升高,P1.05组血浆及肝脏碱性磷酸酶(ALP)、甘油三酯(TG)含量高于其他实验组,而全鱼灰分以及血浆、肝脏总胆固醇(TC)含量呈现相反趋势。P1.05组血浆钙(Ca)含量在各实验组中最高,而磷含量最低,脊椎骨Ca、P含量在P1.05组均低于其他实验组。P1.05组肝脏胰岛素样生长因子1(igf-1)基因表达水平显著高于其他实验组(P<0.05)。综上所述,在本实验条件下,w=1.05%饲料磷水平已满足虎龙杂交斑幼鱼生长的需要,过高磷水平则会导致实验鱼生长下降。The purpose of this study was to investigate the effects of different dietary phosphorus levels on growth,bone mineralization,relatively physiological and biochemical indices in blood and liver of hybrid grouper juveniles.Seven feeding diets with isoenergetic,isoproteic and isolipidic(1421.2 kJ/100 g,47%and 8%,dry matter)were designed.The analyzed dietary phosphorus(P)levels were 1.05%,1.10%,1.20%,1.37%,1.52%,1.59%,and 1.70%,and experimental treatments were abbreviated as P1.05,P1.10,P1.20,P1.37,P1.52,P1.59,and P1.70.The average initial fish weight was(12.14±0.05)g,and experimental fish was hand-fed(8:00 and 16:30)to apparent satiation for 6 weeks.The results showed that the weight gain percentage(WG),specific growth rate(SGR)and condition factor(CF)in P1.05 group were significantly higher than those in other experimental groups(P<0.01),and protein increase value(PPV)was slightly higher in both P1.05 and P1.10 groups than that in the other experimental groups,but there was no significant difference between the groups(P>0.05).With increasing feed phosphorus levels,plasma and liver alkaline phosphatase(ALP)and triglyceride(TG)concentrations were higher in the P1.05 group than those in the other experimental groups,whereas whole fish ash as well as plasma and liver total cholesterol(TC)content showed the opposite trend.Plasma calcium(Ca)content was the highest and P content was the lowest among the experimental groups in the P1.05 group,and vertebral Ca and P contents were lower in the P1.05 group than in the other experimental groups.The expression of liver insulin-like growth factor-1(igf-1)of P1.05 group was also significantly higher than that of other experimental groups(P<0.05).In summary,the 1.05%of dietary phosphorus level can meet the P demand for the growth of hybrid grouper juveniles,and excessive phosphorus supplementations will lead to the decline of fish growth performance.

关 键 词：虎龙杂交斑 饲料磷水平 生长 骨矿化 

分 类 号：S963[农业科学—水产养殖]