花不同发育时期的凉粉草转录组分析及黄酮类合成基因挖掘  

Transcriptome Analysis in Mesona Chinensis Benth at Different Flowering Developmental Stages and Mining of Genes Related to Flavonoid Synthesis

作　　者：尤健开 曾丽珊 王仲冬[1,2] 夏艺萌 林颖 詹若挺[1,2] 张桂芳[1,2] YOU Jiankai;ZENG Lishan;WANG Zhongdong;XIA Yimeng;LIN Ying;ZHAN Ruoting;ZHANGGuifang(School of Chinese Materia Medica,Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China;Key Laboratory of Chinese Medicinal Resource from Lingnan Guangzhou University of Chinese Medicine,Ministry of Education,Guangzhou 510006 Guangdong,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学岭南中药资源教育部重点实验室,广东广州510006

出　　处：《中药新药与临床药理》2024年第11期1772-1780,共9页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家中医药管理局全国中药资源普查项目(GZY-KJS-2018-004);2019年医疗服务与保障能力提升补助资金(中医药事业传承与发展部分)全国中药资源普查项目(财社[2019]39号);广东省省级乡村振兴战略专项资金种业振兴项目—广东省南药种业创新园(2022NJS-00-002、粤财农[2022]184号)。

摘　　要：目的探究凉粉草黄酮类化合物代谢途径的关键基因并比较花不同发育时期(现蕾期、开花期、凋谢期)叶片中的相关基因变化。方法以凉粉草两种栽培品系平远草和台湾草现蕾期、开花期、凋谢期3个时期的叶片为供试材料,运用Illumina NovaSeq 6000测序平台进行转录组测序和生物信息学分析。结果转录数据组装并去冗余得到99945条unigene,其中有56362条unigene成功注释。30116条unigene在KOG数据库中比对获得注释,一般功能预测是最多的一个类群;44461条unigene在GO数据库中比对获得注释,根据功能分为生物进程、细胞组分、分子功能3个GO类别57个小组。筛选得到7466条差异基因,经过KEGG富集分析,差异基因主要富集在植物-病原体相互作用、植物激素信号转导、MAPK信号通路-植物、苯丙烷类生物合成等通路上。51条差异基因与黄酮类生物合成相关,借助基因表达规律的分析,发现两栽培品系基因在同一时期的表达基本一致,现蕾期、开花期与凋谢期3个时期的表达差异显著。随机选取5个差异基因进行RTqPCR验证,结果表明其基因表达与转录组基本一致。结论该研究探知了两种栽培品系中黄酮类化合物代谢途径的关键基因及其在花各发育时期叶片中的表达模式,为探索凉粉草黄酮类生物合成提供了理论基础。Objective To explore the key genes in the flavonoid metabolic pathway of Mesona chinensis Benth and to compare the changes of related genes in leaves at different flower developmental periods(budding,blossoming and withering).Methods Leaves of two cultivars of M.chinensis,Pingyuan grass and Taiwan grass,were used as test materials at three periods(budding,blossoming,and withering),and were subjected to transcriptome sequencing and bioinformatics analyses using the Illumina NovaSeq 6000 sequencing platform.Results A total of 99945 unigenes were obtained from transcript data assembly and de-redundancy,of which 56362 unigenes were successfully annotated.30116 unigenes were compared with the KOG database to obtain annotations,and the general function prediction was the most numerous group.44461 unigenes were compared in the GO database to obtain annotations.These unigenes were classified into three GO categories:biological processes,cellular components,and molecular functions according to the functions,with 57 subgroups.The screening yielded 7466 differentially expressed genes,and KEGG enrichment analysis showed that the differential genes were mainly enriched in the pathways of plant-pathogen interactions,plant hormone signal transduction,MAPK signaling pathway-plant,and phenylpropanoid biosynthesis.A total of 51 differentially expressed genes were related to flavonoid biosynthesis.It was found that the gene expressions of the two cultivars were basically the same in the same period with the help of gene expression analysis,and the expression differences were significant at budding,blossoming and withering stages.Five differential genes were randomly selected for RT-qPCR verification,and the results showed that the gene expression results were basically consistent with the transcriptome expression profile.Conclusion The study identified the key genes of flavonoid metabolism pathway and their expression patterns in the leaves of the two cultivars during the various stages of flower development,which provided a theore

关 键 词：凉粉草 转录组学 差异表达基因 黄酮类化合物 生物合成途径 

分 类 号：R282[医药卫生—中药学]