Effects of growth and starvation conditions on bacterial adhesion to plastic surfaces  

作　　者：Lei He Jianmei Qin Haifeng Rong Meiping Tong 

机构地区：[1]College of Chemical Engineering and Environment,China University of Petroleum-Beijing,Beijing 102249,China [2]The Key Laboratory of Water and Sediment Sciences,Ministry of Education/College of Environmental Sciences and Engineering,Peking University,Beijing 100871,China

出　　处：《National Science Open》2024年第6期152-167,共16页国家科学进展（英文）

基　　金：supported by the National Natural Science Foundation of China(42025706 and 42207424);Science Foundation of China University of Petroleum-Beijing(2462023YJRC020).

摘　　要：The effects of nutrient conditions on the formation of plastisphere were unclear.This study investigated the impacts of growth and starvation conditions on the adhesion of both Gram-negative and Gram-positive bacteria to six plastics in both salt solution and river water.We found that bacteria grown in nutrient-rich condition(LB)contained higher adhesion capability to all six plastics than those grown in nutrient-restricted condition(M9).Starvation process yet decreased bacterial adhesion to plastics under all examined solution conditions.Via deep investigation of the respective contribution of bacterial shape,motility,flagella,and extracellular polymeric substances(EPS)particularly its overall amount,functional groups,major components together with their corresponding secondary structure,as well as its hydrophobicity,the mechanisms driving to the different adhesion capability of bacteria onto plastics subjected to varied nutrient conditions were systemically determined.We found that the enhanced hydrophobicity of bacteria grown in LB contributed to the greater bacterial adhesion to six plastics than those grown in M9.While the changed bacterial shape,the reduced bacteria motility,loss of flagella,and the reduced hydrophobicity of EPS resulted from the change of the composition of proteins and monosaccharides all contributed to the reduced bacteria adhesion to plastics after starvation process.This study indicated that the formation of plastisphere in environment could be greatly influenced by the nutrient condition for bacterial survival,which would affect the environmental risks of plastics.

关 键 词：bacterial adhesion nutrient condition STARVATION plastics extracellular polymeric substance river water 

分 类 号：X52[环境科学与工程—环境工程]