IsDge10 is a hypercompact TnpB nuclease that confers efficient genome editing in rice  

作　　者：Rui Zhang Xu Tang Yao He Yangcun Li Wei Wang Yawei Wang Danning Wang Xuelian Zheng Yiping Qi Yong Zhang 

机构地区：[1]Department of Biotechnology,School of Life Sciences and Technology,Center for Informational Biology,University of Electronic Science and Technology of China,Chengdu 610054,China [2]Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City,Chongqing Key Laboratory of Tree Germplasm Innovation and Utilization,School of Life Sciences,Southwest University,Chongqing 400715,China [3]State Key Lab of Rice Biology,China National Rice Research Institute,Hangzhou 311400 Zhejiang,China [4]Department of Plant Science and Landscape Architecture,University of Maryland,College Park,MD 20742,USA [5]Institute for Bioscience and Biotechnology Research,University of Maryland,Rockville,MD 20850,USA

出　　处：《Plant Communications》2024年第11期6-9,共4页植物通讯（英文）

基　　金：Biological Breeding-Major Projects(2023ZD04076)awarded to X.T.and Y.Z.;National Natural Science Foundation of China(award nos.32270433,32101205,and 32072045)to Y.Z.,X.Z.;X.T.It was also supported by the NSF Plant Genome Research Program(IOS-2029889 and IOS-2132693)to Y.Q.

摘　　要：Dear Editor,Cas9 and Cas12a have been widely applied in genome engineering in both plant and human cells(Tang and Zhang,2023).However,the relatively large sizes restrict their delivery into cells via viral vectors.As hypothetical ancestors of Cas9 and Cas12a,IscB and TnpB have been reported as RNA-guided DNA endonucleases suitable for genome editing in human cells(Han et al.,2023;Xiang et al.,2023).More recently,a eukaryotic RNA-guided endonuclease named Fanzor has demonstrated genome editing capabilities in human cells(Saito et al.,2023).These nucleases,such as IsDge10,are significantly smaller(∼390 amino acids for IsDge10)compared to Cas9 and Cas12a(e.g.,∼1300 amino acids for SpCas9).However,the applicability of TnpB,IscB,or Fanzor for plant genome engineering remained unexplored.Here,we evaluated a series of nucleases from the TnpB,IscB,and Fanzor families and successfully developed a miniature plant genome editor using IsDge10,a TnpB nuclease from Deinococcus geothermalis.

关 键 词：SIZES restrict compact 

分 类 号：Q943.2[生物学—植物学]