An integrated pipeline facilitates fast cloning of a new powdery mildew resistance gene from the wheat wild relative Aegilops umbellulata  

作　　者：Huagang He Jiale Wang Jiabao Liang Qianyuan Zhang Minfeng Xue Zhaozhao Chen Qiulian Tang Xiaobei Chen Shanying Zhu Yajun Wang 

机构地区：[1]School of Life Sciences,Jiangsu University,Zhenjiang,Jiangsu 212013,China [2]Key Laboratory of Plant Design,National Key Laboratory of Plant Molecular Genetics,Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China [3]Institute of Plant Protection and Soil Science,Hubei Academy of Agricultural Sciences,Wuhan 430064,China [4]School of Environment and Safety Engineering,Jiangsu University,Zhenjiang,Jiangsu 212013,China [5]These authors contributed equally to this article

出　　处：《Plant Communications》2024年第11期10-13,共4页植物通讯（英文）

基　　金：Natural Science Foundation of Jiangsu Province(BK20231321);National Natural Science Foundation of China(32171990);State Key Laboratory of Plant Cell and Chromosome Engineering(PCCE-KF-2022-07).

摘　　要：Dear Editor,Bread wheat(Triticum aestivum L.,2n=6x=42,AABBDD)is a major cereal crop consumed by approximately 30%of the global population.Wheat production faces continuous challenges from various fungal diseases,including powdery mildew,rusts,and Fusarium head blight(Singh et al.,2016).Aegilops umbellulata(Ae.umbellulate,2n=2x=14,UU),a diploid species within the genus Aegilops and closely related to cultivated bread wheat,exhibits resistance to several wheat pathogens,making it a valuable source of disease resistance genes for wheat improvement.Notably,several resistance genes from Ae.umbellulata have been successfully transferred into bread wheat,including the powdery mildew resistance gene PmY39(Zhu et al.,2006),the leaf rust resistance genes Lr9(Sears,1956)and Lr76(Bansal et al.,2020),and the stripe rust resistance gene Yr70(Bansal et al.,2020).Here,we investigate the responses of a panel of Ae.umbellulata accessions to Blumeria graminis f.sp.tritici(Bgt),the pathogen causing wheat powdery mildew,and report the cloning of the powdery mildew resistance gene PmAeu1 through an integrated approach combining bulked segregant RNA sequencing(BSR-seq),fine genetic mapping,low-depth PacBio sequencing,and functional validation via stable transformation in wheat.

关 键 词：MILDEW AEGILOPS blight 

分 类 号：Q943.2[生物学—植物学] S512[农业科学—作物学]