The abscisic acid-responsive transcriptional regulatory module CsERF110-CsERF53 orchestrates citrus fruit coloration  

作　　者：Quan Sun Zhengchen He Di Feng Ranran Wei Yingzi Zhang Junli Ye Lijun Chai Juan Xu Yunjiang Cheng Qiang Xu Xiuxin Deng 

机构地区：[1]National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops,Huazhong Agricultural University,Wuhan 430070,China [2]National Research Center for Apple Engineering and Technology,Shandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient Production,College of Horticulture Science and Engineering,Shandong Agricultural University,Taian,Shandong 271018,China [3]Hubei Hongshan Laboratory Wuhan,Hubei 430070,China [4]These authors contributed equally to this article

出　　处：《Plant Communications》2024年第11期157-172,共16页植物通讯（英文）

基　　金：National Key R&D Program of China(2023YFD2300600);National Natural Science Foundation of China(no.31930095);National Modern Agricultural(Citrus)Technology Systems of China(no.CARS-27).

摘　　要：Carotenoid biosynthesis is closely associated with abscisic acid(ABA)during the ripening process of non-climacteric fruits,but the regulatory mechanism that links ABA signaling to carotenoid metabolism remains largely unclear.Here,we identified two master regulators of ABA-mediated citrus fruit coloration,CsERF110 and CsERF53,which activate the expression of carotenoid metabolism genes(CsGGPPS,CsPSY,CsPDS,CsCRTISO,CsLCYB2,CsLCYE,CsHYD,CsZEP,and CsNCED2)to facilitate carotenoid accumulation.Further investigations showed that CsERF110 not only activates the expression of CsERF53 by binding to its promoter but also interacts with CsERF53 to form the transcriptional regulatory module CsERF110-CsERF53.We also discovered a positive feedback regulatory loop between the ABA signal and carotenoid metabolism regulated by the transcriptional regulatory module CsERF110-CsERF53.Our results reveal that the CsERF110-CsERF53 module responds to ABA signaling,thereby orchestrating citrus fruit coloration.Considering the importance of carotenoid content for citrus and many other carotenoid-rich crops,the revelation of molecular mechanisms that underlie ABA-mediated carotenoid biosynthesis in plants will facilitate the development of transgenic/gene-editing approaches,further contributing to improving the quality of citrus and other carotenoid-rich crops.

关 键 词：citrus abscisic acid ABA fruit coloration carotenoid metabolism ethylene responsive factor ERF transcriptional regulatory module 

分 类 号：Q94[生物学—植物学]