Diagnosing and reintegrating traceability of infectious diseases via metagenomic next-generation sequencing:Study of a severe case of Rickettsia japonica infection  

作　　者：Zhenhua Lu Jing Han Yuanyuan Wang Zhen He Shuxuan Song Kun Liu Zhongjun Shao 

机构地区：[1]Department of Epidemiology,Ministry of Education Key Laboratory of Hazard Assessment and Control in Special Operational Environment,School of Public Health,Air Force Medical University,Xi’an 710032,China [2]Department of Infectious Diseases,Tangdu Hospital,Air Force Medical University,Xi’an 710038,China

出　　处：《Infectious Medicine》2024年第1期79-83,共5页感染医学（英文）

基　　金：supported by the National Natural Science Foundation of China(82273689);Key Research and Development Program of Shaanxi(2024SF-YBXM-289).

摘　　要：Background:In this study,we present a case of Japanese spotted fever(JSF)caused by Rickettsia japonica and use this case to investigate the process of diagnosing and reintegrating traceability of infectious diseases via metagenomic next-generation sequencing(mNGS).Methods:From data relating to epidemiological history,clinical and laboratory examinations,and mNGS se-quencing,a diagnosis of severe JSF was concluded.Results:A detailed field epidemiological investigation discovered parasitic Haemaphysalis longicornis from a host animal(dog)in the domicile of the patient,within which R.japonica was detected,along with a diverse array of other potentially pathogenic microorganisms that could cause other infectious diseases.Conclusion:The mNGS provided an efficient method to diagnose JSF infection.This methodology could also be applied to field epidemiological investigations to establish the traceability of infectious diseases.

关 键 词：Rickettsial disease Japanese spotted fever mNGS Field epidemiological investigation 

分 类 号：R511[医药卫生—内科学]