3-D bioprinted human-derived skin organoids accelerate full-thickness skin defects repair  

作　　者：Tao Zhang Shihao Sheng Weihuang Cai Huijian Yang Jiameng Li Luyu Niu Wanzhuo Chen Xiuyuan Zhang Qirong Zhou Chuang Gao Zuhao Li Yuanwei Zhang Guangchao Wang Hao Shen Hao Zhang Yan Hu Zhifeng Yin Xiao Chen Yuanyuan Liu Jin Cui Jiacan Su 

机构地区：[1]Department of Orthopedics,Xinhua Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200433,China [2]Department of Orthopedics,First Affiliated Hospital,Naval Medical University,Shanghai,200433,China [3]School of Mechatronic Engineering and Automation,Shanghai University,Shanghai,200444,China [4]Department of Laboratory Medicine,Shanghai Zhongye Hospital,Shanghai,200941,China [5]Institute of Translational Medicine,Shanghai University,Shanghai,200444,China [6]Trauma Orthopedics Center,Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,200092,China [7]Institute of Musculoskeletal Injury and Translational Medicine of Organoids,Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,200092,China [8]Department of Orthopedics,Shanghai Zhongye Hospital,Shanghai,200941,China [9]Wenzhou Institute of Shanghai University,Wenzhou,325000,China [10]Institute of Biomedicine,Shanghai University,Shanghai,200444,China [11]National Center for Translational Medicine(Shanghai),SHU Branch,Shanghai University,Shanghai,200444,China

出　　处：《Bioactive Materials》2024年第12期257-269,共13页生物活性材料（英文）

基　　金：Science Foundation of China(NO.92249303,NO.82230071,No.82172098,No.82371603);Jiangsu Province Natural Science and Technological Project(No.BK20231218);Experimental Animal Research Field Project Shanghai Science and Technology Commission(No.23141900600);Research Physician Innovation and Transformation Ability Training Project from Shanghai Health Commission(No.SHDC2023CRT013);Basic Medical Innovation Project of Changhai Hospital(No.20237Y38);Deep Blue Talent Project of Naval Medical University(Jin Cui);Shanghai Oriental Talent Program(Xiao Chen,Yuanyuan Liu).

摘　　要：The healing of large skin defects remains a significant challenge in clinical settings.The lack of epidermal sources,such as autologous skin grafting,limits full-thickness skin defect repair and leads to excessive scar formation.Skin organoids have the potential to generate a complete skin layer,supporting in-situ skin regeneration in the defect area.In this study,skin organoid spheres,created with human keratinocytes,fibroblasts,and endothelial cells,showed a specific structure with a stromal core surrounded by surface keratinocytes.We selected an appropriate bioink and innovatively combined an extrusion-based bioprinting technique with dual-photo source cross-linking technology to ensure the overall mechanical properties of the 3D bioprinted skin organoid.Moreover,the 3D bioprinted skin organoid was customized to match the size and shape of the wound site,facilitating convenient implantation.When applied to full-thickness skin defects in immunodeficient mice,the 3D bioprinted human-derived skin organoid significantly accelerated wound healing through in-situ regeneration,epithelialization,vascularization,and inhibition of excessive inflammation.The combination of skin organoid and 3D bioprinting technology can overcome the limitations of current skin substitutes,offering a novel treatment strategy to address large-area skin defects.

关 键 词：Skin organoid Skin defect 3D bioprinting Wound healing 

分 类 号：R318[医药卫生—生物医学工程]