Baiting bacteria with amino acidic and peptidic corona coated defect-engineered antimicrobial nanoclusters for optimized wound healing  

作　　者：Maonan Wang Houjuan Zhu Yuling Xue Yanxia Duan Hua Tian Qi Liu Yuzhu Zhang Zibiao Li Xian Jun Loh Enyi Ye Gang Yin Xuemei Wang Xianguang Ding David Tai Leong 

机构地区：[1]Department of Chemical and Biomolecular Engineering,Faculty of Engineering,National University of Singapore,Singapore,117585,Singapore [2]Department of Pathology,Xiangya Hospital,School of Basic Medical Sciences,Central South University,Changsha,China [3]Institute of Materials Research and Engineering(IMRE),Agency for Science,Technology and Research(A*STAR),2 Fusionopolis Way,Innovis#08-03,Singapore,138634,Singapore [4]State Key Laboratory of Bioelectronics(Chien-Shiung Wu Lab),School of Biological Science and Medical Engineering,Southeast University,Nanjing,210096,China [5]Key Laboratory for Organic Electronics and Information Displays&Jiangsu Key Laboratory for Biosensors,Nanjing University of Posts&Telecommunications,Nanjing,210023,China

出　　处：《Bioactive Materials》2024年第12期628-643,共16页生物活性材料（英文）

基　　金：the funding provided by the National University of Singapore Reimagine Grant(A-0009179-02-00,A-0009179-03-00);National Natural Science Foundation of China(82303841);Hunan Provincial Natural Science Foundation(2023JJ40800);the program of China Scholarships Council(No.202006090323).

摘　　要：Keeping steps ahead of the bacteria in the race for more efficacious antibacterial strategies is increasingly difficult with the advent of bacterial resistance genes.Herein,we engineered copper sulfide nanoclusters(CuS_(x) NCs)with variable sulfur defects for enhanced dual-treatment of bacterial infections by manipulating photothermal effects and Fenton-like activity.Next,by encasing CuS_(x) NCs with a complex mixture of amino acids and short peptides derived from Luria-Bertani bacterial culture media as a protein corona,we managed to coax E.Coli to take up these CuS_(x) NCs.As a whole,Amino-Pep-CuS_(x) NCs was perceived as a food source and actively consumed by bacteria,enhancing their effective uptake by at least 1.5-fold greater than full length BSA protein BSA-corona CuS_(x) NCs.Through strategically using defect-engineering,we successfully fine-tune photothermal effect and Fenton-like capacity of CuS_(x) NCs.Increased sulfur defects lead to reduced but sufficient heat generation under solar-light irradiation and increased production of toxic hydroxyl radicals.By fine-tuning sulfur defects during synthesis,we achieve CuS_(x) NCs with an optimal synergistic effect,significantly enhancing their bactericidal properties.These ultra-small and biodegradable CuS_(x) NCs can rapidly break down after treatment for clearance.Thus,Amino-Pep-CuS_(x) NCs demonstrate effective eradication of bacteria both in vitro and in vivo because of their relatively high uptake,optimal balanced photothermal and chemodynamic outcomes.Our study offers a straightforward and efficient method to enhance bacterial uptake of next generation of antibacterial agents.

关 键 词：Defect-engineered Photothermal effect Chemodynamic effect Copper sulfide nanoclusters Protein corona 

分 类 号：O62[理学—有机化学]