Injectable and rapidly expandable thrombin-decorated cryogels achieve rapid hemostasis and high survival rates in a swine model of lethal junctional hemorrhage  

作　　者：Syed Muntazir Andrabi S.M.Shatil Shahriar Al-Murtadha Al-Gahmi Benjamin L.Wilczewski Mark A.Carlson Jingwei Xie 

机构地区：[1]Department of Surgery-Transplant and Mary&Dick Holland Regenerative Medicine Program,University of Nebraska Medical Center,Omaha,NE,68198,USA [2]Departments of Surgery-General Surgery and Genetics,Cell Biology and Anatomy,University of Nebraska Medical Center,Omaha,NE,68198,USA [3]Department of Mechanical and Materials Engineering,University of Nebraska Lincoln,Lincoln,NE,68588,USA

出　　处：《Bioactive Materials》2024年第8期154-168,共15页生物活性材料（英文）

基　　金：supported by startup funds from the University of Nebraska Medical Center(UNMC)and Congressionally Directed Medical Research Program(CDMRP)/Peer Reviewed Medical Research Program(PRMRP)FY19 W81XWH2010207 to J.X.;M.A.C.We thank the Proteomics&Metabolomics Facility(RRID:SCR_021314),Nebraska Center for Biotechnology at the University of Nebraska-Lincoln for the mass spectrometry analysis.The facility and instrumentation are supported by the Nebraska Research Initiative.

摘　　要：Effective therapies are urgently needed to stabilize patients with marginally compressible junctional hemorrhage long enough to get them to the hospital alive.Herein,we report injectable and rapidly expandable cryogels consisting of polyacrylamide and thrombin(AT cryogels)created by cryo-polymerization for the efficient management of lethal junctional hemorrhage in swine.The produced cryogels have small pore sizes and highly interconnected porous architecture with robust mechanical strength.The cryogels exhibit rapid shape memory properties and prove to be resilient against fatigue.These cryogels also show high water/blood absorption capacity,fast blood clotting effect,and enhanced adhesion of red blood cells and platelets in vitro.Further,in vivo,hemostatic efficacy tests in a lethal swine junctional hemorrhage model suggest that treatment with AT cryogels,especially AT-2 cryogels,achieves the least blood loss and the highest survival rate(100%)compared to currently employed products such as XStat®and combat gauze.The high hemostatic performance of the cryogels may be attributed to highly interconnected porous architecture with small pore size and the use of thrombin as a pro-coagulant agent.Collectively,injectable and rapidly expandable thrombin-decorated polyacrylamide-based cryogels show significant promise as hemostatic material,offering effective management of marginally compressible junctional hemorrhages in prehospital settings.

关 键 词：Polyacrylamide cryogels Blood clotting Shape memory and injectable Lethal junctional hemorrhage 

分 类 号：R318[医药卫生—生物医学工程]