Diatomite-incorporated hierarchical scaffolds for osteochondral regeneration  

作　　者：Cuijun Deng Chen Qin Zhenguang Li Laiya Lu Yifan Tong Jiaqi Yuan Feng Yin Yu Cheng Chengtie Wu 

机构地区：[1]Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation,Clinical Research Center for Anesthesiology and Perioperative Medicine,Translational Research Institute of Brain and Brain-Like Intelligence,Shanghai Fourth People’s Hospital,School of Medicine,Tongji University,Shanghai,200434,PR China [2]State Key Laboratory of High Performance Ceramics and Superfine Microstructure,Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai,200050,PR China [3]Department of Joint Surgery,Shanghai East Hospital,School of Medicine,Tongji University,150 Jimo Road,Shanghai,200032,PR China [4]State Key Laboratory of Molecular Engineering of Polymers,Department of Macromolecular Science,Fudan University,Shanghai,200438,PR China

出　　处：《Bioactive Materials》2024年第8期305-320,共16页生物活性材料（英文）

基　　金：The National Key Research and Development Program of China(2023YFB3813000);the National Natural Science Foundation of China(32271441,82102196,32225028);the Natural Science Foundation of Shanghai(21DZ1205600,22ZR1462800);Shanghai Municipal Education Commission Innovative Program(2023ZKZD27);State Key Laboratory of Molecular Engineering of Polymers(Fudan University,K2021-12).

摘　　要：Osteochondral regeneration involves the highly challenging and complex reconstruction of cartilage and subchondral bone.Silicon(Si)ions play a crucial role in bone development.Current research on Si ions mainly focuses on bone repair,by using silicate bioceramics with complex ion compositions.However,it is unclear whether the Si ions have important effect on cartilage regeneration.Developing a scaffold that solely releases Si ions to simultaneously promote subchondral bone repair and stimulate cartilage regeneration is critically important.Diatomite(DE)is a natural diatomaceous sediment that can stably release Si ions,known for its abundant availability,low cost,and environmental friendliness.Herein,a hierarchical osteochondral repair scaffold is uniquely designed by incorporating gradient DE into GelMA hydrogel.The adding DE microparticles provides a specific Si source for controlled Si ions release,which not only promotes osteogenic differentiation of rBMSCs(rabbit bone marrow mesenchymal stem cells)but also enhances proliferation and maturation of chondrocytes.Moreover,DE-incorporated hierarchical scaffolds significantly promoted the regeneration of cartilage and subchondral bone.The study suggests the significant role of Si ions in promoting cartilage regeneration and solidifies their foundational role in enhancing bone repair.Furthermore,it offers an economic and eco-friendly strategy for developing high value-added osteochondral regenerative bioscaffolds from low-value ocean natural materials.

关 键 词：DIATOMITE Si release Hierarchical scaffolds 3D printing Osteochondral regeneration 

分 类 号：R318[医药卫生—生物医学工程]