Enhancing volumetric muscle loss(VML)recovery in a rat model using super durable hydrogels derived from bacteria  

作　　者：Seyyed Vahid Niknezhad Mehdi Mehrali Farinaz Riyahi Khorasgani Reza Heidari Firoz Babu Kadumudi Nasim Golafshan Miguel Castilho Cristian Pablo Pennisi Masoud Hasany Mohammadjavad Jahanshahi Mohammad Mehrali Younes Ghasemi Negar Azarpira Thomas L.Andresen Alireza Dolatshahi-Pirouz 

机构地区：[1]Burn and Wound Healing Research Center,Shiraz University of Medical Sciences,Shiraz,71987-54361,Iran [2]Department of Civil and Mechanical Engineering,Technical University of Denmark,2800,Kgs Lyngby,Denmark [3]Department of Health Technology,Technical University of Denmark,2800,Kongens Lyngby,Denmark [4]Pharmaceutical Sciences Research Center,Shiraz University of Medical Sciences,Shiraz,Iran [5]Department of Orthopaedics,University Medical Center Utrecht,Utrecht,3584 CX,the Netherlands [6]Department of Biomedical Engineering,Eindhoven University of Technology,the Netherlands [7]Regenerative Medicine Group,Department of Health Science and Technology,Aalborg University,9260,Gistrup,Denmark [8]Department of Chemistry,Faculty of Science,University of Jiroft,8767161167,Jiroft,Iran [9]Faculty of Engineering Technology,Department of Thermal and Fluid Engineering(TFE),University of Twente,7500 AE,Enschede,the Netherlands [10]Transplant Research Center,Shiraz University of Medical Sciences,Shiraz,Iran [11]Institute for Complex Molecular Systems,Eindhoven University of Technology,Eindhoven,the Netherlands [12]Department of Health Technology,Section for Biotherapeutic Engineering and Drug Targeting,Technical University of Denmark,2800,Kongens Lyngby,Denmark

出　　处：《Bioactive Materials》2024年第8期540-558,共19页生物活性材料（英文）

基　　金：the Danish Council for Independent Research(Technology and Production Sciences,8105-00003B);Denmark,the Novo Nordisk Foundation(NNF22OC0079994 in the call"Project Grants in the Natural and Technical Sciences 2022);Denmark,and the VIDI research programme with project number R0004387;which is(partly)financed by The Netherlands Organization for Scientific Research(NWO),The Netherlands.This work has also received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no.951747;Masoud Hasany and Mehdi Mehrali would like to acknowledge the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 899987.

摘　　要：Bacteria can be programmed to deliver natural materials with defined biological and mechanical properties for controlling cell growth and differentiation.Here,we present an elastic,resilient and bioactive polysaccharide derived from the extracellular matrix of Pantoea sp.BCCS 001.Specifically,it was methacrylated to generate a new photo crosslinkable hydrogel that we coined Pantoan Methacrylate or put simply PAMA.We have used it for the first time as a tissue engineering hydrogel to treat VML injuries in rats.The crosslinked PAMA hydrogel was super elastic with a recovery nearing 100%,while mimicking the mechanical stiffness of native muscle.After inclusion of thiolated gelatin via a Michaelis reaction with acrylate groups on PAMA we could also guide muscle progenitor cells into fused and aligned tubes-something reminiscent of mature muscle cells.These results were complemented by sarcomeric alpha-actinin immunostaining studies.Importantly,the implanted hydrogels exhibited almost 2-fold more muscle formation and 50%less fibrous tissue formation compared to untreated rat groups.In vivo inflammation and toxicity assays likewise gave rise to positive results confirming the biocompatibility of this new biomaterial system.Overall,our results demonstrate that programmable polysaccharides derived from bacteria can be used to further advance the field of tissue engineering.In greater detail,they could in the foreseeable future be used in practical therapies against VML.

关 键 词：HYDROGEL simply FUSED 

分 类 号：O63[理学—高分子化学]