Tailored endothelialization enabled by engineered endothelial cell vesicles accelerates remodeling of small-diameter vascular grafts  

作　　者：Zihao Wang Mengxue Zhou Mengyu Li Jinyu Li Shengmin Zhang Jianglin Wang 

机构地区：[1]Department of Biomedical Engineering,Huazhong University of Science and Technology,Wuhan,430074,China [2]NMPA Research Base of Regulatory Science for Medical Devices,Institute of Regulatory Science for Medical Devices,Huazhong University of Science and Technology,Wuhan,430074,China

出　　处：《Bioactive Materials》2024年第11期127-136,共10页生物活性材料（英文）

基　　金：National Key Research and Development Program of China(2022YFA1105100);National Natural Science Foundation of China(32301102;32171323);Fundamental Research Funds for the Central Universities(YCJJ20230215);Science,Technology and Innovation Commission of Shenzhen Municipality(KCXFZ20211020164544008);Sanming Project of Medicine in Shenzhen(SZSM201812055).

摘　　要：Current gold standard for the replacement of small-diameter blood vessel(ID<4 mm)is still to utilize the autologous vessels of patients due to the limitations of small-diameter vascular grafts(SDVG)on weak endothelialization,intimal hyperplasia and low patency.Herein,we create the SDVG with the tailored endothelialization by applying the engineered endothelial cell vesicles to camouflaging vascular grafts for the enhancement of vascular remodeling.The engineered endothelial cell vesicles were modified with azide groups(ECVs-N3)through metabolic glycoengineering to precisely link the vascular graft made of PCL-DBCO via click chemistry,and thus fabricating ECVG(ECVs-N3 modified SDVG),which assists inhibition of platelet adhesion and activation,promotion of ECs adhesion and enhancement of anti-inflammation.Furthermore,In vivo single-cell transcriptome analysis revealed that the proportion of ECs in the cell composition of ECVG surpassed that of PCL,and the tailored endothelialization enabled to convert endothelial cells(ECs)into some specific ECs clusters.One of the specific cluster,Endo_C5 cluster,was only detected in ECVG.Consequently,our study integrates the engineered membrane vesicles of ECVs-N3 from native ECs for tailored endothelialization on SDVG by circumventing the limitations of living cells,and paves a new way to construct the alternative endothelialization in vessel remodeling following injury.

关 键 词：Small-diameter vascular grafts Tailored endothelialization Endothelial cell vesicles Click chemistry Vascular remodeling 

分 类 号：O62[理学—有机化学]