Injectable immunoregulatory hydrogels sequentially drive phenotypic polarization of macrophages for infected wound healing  

作　　者：Yuxiang Wang Chen Zhou Zhulian Li Gong Li Yaping Zou Xing Li Peiyang Gu Jingyi Liu Lang Bai Hong Yan Jie Liang Xingdong Zhang Yujiang Fan Yong Sun 

机构地区：[1]National Engineering Research Center for Biomaterials,Sichuan University,29#Wangjiang Road,Chengdu,Sichuan,610064,China [2]College of Biomedical Engineering,Sichuan University,29#Wangjiang Road,Chengdu,Sichuan,610064,China [3]Center of Infectious Diseases,West China Hospital of Sichuan University,37#Guoxue Lane,Chengdu,Sichuan,610041,China [4]Department of Plastic,Aesthetic,Reparative and Reconstructive Surgery/Wound Repair Center,West China Second University Hospital of Sichuan University,20#Section 3,South Renmin Road,Chengdu,Sichuan,610041,China [5]Sichuan Testing Center for Biomaterials and Medical Devices,Sichuan University,29#Wangjiang Road,Chengdu 610064,China

出　　处：《Bioactive Materials》2024年第11期193-206,共14页生物活性材料（英文）

基　　金：National Key R&D Project of China(No.2022YFC2401800);National Natural Science Foundation of China(32071352 and 32271419).

摘　　要：Regulating macrophage phenotypes to reconcile the conflict between bacterial suppression and tissue regeneration is ideal for treating infectious skin wounds. Here, an injectable immunoregulatory hydrogel (SrmE20) that sequentially drives macrophage phenotypic polarization (M0 to M1, then to M2) was constructed by integrating anti-inflammatory components and proinflammatory solvents. In vitro experiments demonstrated that the proinflammatory solvent ethanol stabilized the hydrogel structure, maintained the phenolic hydroxyl group activity, and achieved macrophages' proinflammatory transition (M0 to M1) to enhance antibacterial effects. With ethanol depletion, the hydrogel's cations and phenolic hydroxyl groups synergistically regulated macrophages' anti-inflammatory transition (M1 to M2) to initiate regeneration. In the anti-contraction full-thickness wound model with infection, this hydrogel effectively eliminated bacteria and even achieved anti-inflammatory M2 macrophage accumulation at three days post-surgery, accelerated angiogenesis and collagen deposition. By sequentially driving macrophage phenotypic polarization, this injectable immunoregulatory hydrogel will bring new guidance for the care and treatment of infected wounds.

关 键 词：Sequential immunoregulation Macrophage phenotypic polarization Injectable hydrogel Infected wounds 

分 类 号：O62[理学—有机化学]