Personalized composite scaffolds for accelerated cell-and growth factor-free craniofacial bone regeneration  

作　　者：Mirae Kim Xinlong Wang Yiming Li Zitong Lin Caralyn P.Collins Yugang Liu Yujin Ahn Hsiu-Ming Tsal Joseph W.Song Chongwen Duan Yi Zhu Cheng Sun Tong-Chuan He Yuan Luo Russell R.Reid Guillermo A.Ameer 

机构地区：[1]Department of Biomedical Engineering,Northwestern University,Evanston,IL,60208,USA [2]Center for Advanced Regenerative Engineering,Northwestern University,Evanston,IL,60208,USA [3]Department of Preventive Medicine,Feinberg School of Medicine,Northwestern University,Chicago,IL,60611,USA [4]Department of Mechanical Engineering,Northwestern University,Evanston,IL,60208 USA [5]Department of Chemical and Biomolecular Engineering,University of Illinois at Urbana-Champaign,Urbana,IL,61820,USA [6]Department of Radiology,The University of Chicago,Chicago,IL,60637,USA [7]Molecular Oncology Laboratory,Department of Orthopedic Surgery and Rehabilitation Medicine,The University of Chicago Medical Center,Chicago,IL,60637,USA [8]Clinical and Translational Sciences Institute,Northwestern University,Chicago,IL,60611,USA [9]Center for Collaborative AI in Healthcare,Institute for AI in Medicine,Northwestern University,Chicago,IL,60611,USA [10]Laboratory of Craniofacial Biology and Development,Section of Plastic and Reconstructive Surgery,Department of Surgery,The University of Chicago Medical Center,Chicago,IL,60637,USA [11]Department of Surgery,Feinberg School of Medicine,Northwestern University,Chicago,IL,60611,USA [12]Chemistry of Life Process Institute,Northwestern University,Chicago,IL,60208,USA [13]International Institute for Nanotechnology,Northwestern University,Evanston,IL,60208,USA

出　　处：《Bioactive Materials》2024年第11期427-439,共13页生物活性材料（英文）

基　　金：National Research Foundation of Korea(2021R1A6A3A14039205)(Mirae Kim);National Institutes of Health/National Institute of Dental and Craniofacial Research(R01DE030480)(Russell R.Reid).

摘　　要：Approaches to regenerating bone often rely on integrating biomaterials and biological signals in the form of cells or cytokines.However,from a translational point of view,these approaches are challenging due to the sourcing and quality of the biologic,unpredictable immune responses,complex regulatory paths,and high costs.We describe a simple manufacturing process and a material-centric 3D-printed composite scaffold system(CSS)that offers distinct advantages for clinical translation.The CSS comprises a 3D-printed porous polydiolcitrate-hydroxyapatite composite elastomer infused with a polydiolcitrate-graphene oxide hydrogel composite.Using a micro-continuous liquid interface production 3D printer,we fabricate a precise porous ceramic scaffold with 60 wt%hydroxyapatite resembling natural bone.The resulting scaffold integrates with a thermoresponsive hydrogel composite in situ to fit the defect,which is expected to enhance surface contact with surrounding tissue and facilitate biointegration.The antioxidative properties of citrate polymers prevent long-term inflammatory responses.The CSS stimulates osteogenesis in vitro and in vivo.Within 4 weeks in a calvarial critical-sized bone defect model,the CSS accelerated ECM deposition(8-fold)and mineralized osteoid(69-fold)compared to the untreated.Through spatial transcriptomics,we demonstrated the comprehensive biological processes of CSS for prompt osseointegration.Our material-centric approach delivers impressive osteogenic properties and streamlined manufacturing advantages,potentially expediting clinical application for bone reconstruction surgeries.

关 键 词：Craniofacial bone regeneration Composite scaffold Material-centric approach3D printing Citrate biomaterial 

分 类 号：R318[医药卫生—生物医学工程]