A biodegradable microgrooved and tissue mechanocompatible citrate-based scaffold improves bladder tissue regeneration  

作　　者：Madeleine Goedegebuure Matthew I.Bury Xinlong Wang Pasquale Sanfelice Federico Cammarata Larry Wang Tiffany T.Sharma Nachiket Rajinikanth Vikram Karra Vidhika Siddha Arun K.Sharma Guillermo A.Ameer 

机构地区：[1]Department of Biomedical Engineering,Northwestern University,Evanston,IL,USA [2]Department of Surgery,Division of Urology,Lurie Children's Hospital of Chicago,Chicago,IL,USA [3]Department of Urology,Feinberg School of Medicine,Northwestern University,Chicago,IL,USA [4]Department of Surgery,Feinberg School of Medicine,Northwestern University,Chicago,IL,USA [5]Center for Advanced Regenerative Engineering,Northwestern University,Chicago,IL,USA [6]International Institute for Nanotechnology,Northwestern University,Chicago,IL,USA [7]Simpson Querrey Institute,Northwestern University,Chicago,IL,USA [8]Chemistry for Life Processes Institute,Northwestern University,Chicago,IL,USA [9]Stanley Manne Children's Research Institute,Lurie Children's Hospital of Chicago,Chicago,IL,USA

出　　处：《Bioactive Materials》2024年第11期553-563,共11页生物活性材料（英文）

基　　金：Center for Advanced Regenerative Engineering and the Department of Urology at the Feinberg School of Medicine at Northwestern University,the Division of Urology at Lurie Children's Hospital of Chicago;NIH grant R01EB026575 to A.K.S.and G.A.A,NIH grant R01DK109539 to A.K.S.,and T32 grant(EB031527)to G.A.A.

摘　　要：Chronic bladder dysfunction due to bladder disease or trauma is detrimental to affected patients as it can lead to increased risk of upper urinary tract dysfunction. Current treatment options include surgical interventions that enlarge the bladder with autologous bowel tissue to alleviate pressure on the upper urinary tract. This highly invasive procedure, termed bladder augmentation enterocystoplasty (BAE), significantly increases the risk of patient morbidity and mortality due to the incompatibility between bowel and bladder tissue. Therefore, patients would significantly benefit from an alternative treatment strategy that can regenerate healthy tissue and restore overall bladder function. Previous research has demonstrated the potential of citrate-based scaffolds co-seeded with bone marrow-derived stem/progenitor cells as an alternative graft for bladder augmentation. Recognizing that contact guidance can potentially influence tissue regeneration, we hypothesized that microtopographically patterned scaffolds would modulate cell responses and improve overall quality of the regenerated bladder tissue. We fabricated microgrooved (MG) scaffolds using the citrate-based biomaterial poly (1,8-octamethylene-citrate-co-octanol) (POCO) and co-seeded them with human bone marrow-derived mesenchymal stromal cells (MSCs) and CD34+ hematopoietic stem/progenitor cells (HSPCs). MG POCO scaffolds supported MSC and HSPC attachment, and MSC alignment within the microgrooves. All scaffolds were characterized and assessed for bladder tissue regeneration in an established nude rat bladder augmentation model. In all cases, normal physiological function was maintained post-augmentation, even without the presence of stem/progenitor cells. Urodynamic testing at 4-weeks post-augmentation for all experimental groups demonstrated that bladder capacity increased and bladder compliance was normal. Histological evaluation of the regenerated tissue revealed that cell-seeded scaffolds restored normal bladder smooth muscle content and resulted i

关 键 词：Guided bladder tissue regeneration Mesenchymal stem cell Hematopoietic stem cell MICROTOPOGRAPHY NEOVASCULARIZATION 

分 类 号：R318[医药卫生—生物医学工程]