Expansion Strategy-Driven Micron-Level Resolution Mass Spectrometry Imaging of Lipids in Mouse Brain Tissue  

作　　者：Yik Ling Winnie Hung Chengyi Xie Jianing Wang Xin Diao Ruxin Li Xiaoxiao Wang Shulan Qiu Jiacheng Fang Zongwei Cai 

机构地区：[1]State Key Laboratory of Environmental and Biological Analysis,Hong Kong Baptist University,Hong Kong 999077 [2]Department of Chemistry,Hong Kong Baptist University,Hong Kong 999077 [3]Department of Health Technology and Informatics,The Hong Kong Polytechnic University(PolyU),Hong Kong 999077

出　　处：《CCS Chemistry》2024年第11期2662-2670,共9页中国化学会会刊（英文）

基　　金：supported by the General Research Fund(grant no.12302122)of the Research Grants Council;Hong Kong Special Administrative Region,China,the Start-up Grant from Hong Kong Baptist University,and the State Key Laboratory of Environmental and Biological Analysis Research Grant(grant no.SKLP_2021_P04)。

摘　　要：A novel method for enhanced resolution,termed expansion mass spectrometry imaging,has been developed for lipid mass spectrometry imaging,utilizing existing commercially available mass spectrometers without necessitating modifications.This approach involves embedding tissue sections in a swellable polyelectrolyte gel,with the target biomolecules indirectly anchored to the gel network.By employing matrix-assisted laser desorption ionization mass spectrometry imaging,the method has realized an enhanced spatial resolution that surpasses the conventional resolution limits of commercial instruments by approximately 4.5 fold.This enhancement permits the detailed visualization of intricate structures within the mouse brain at a subcellular level,with a lateral resolution nearing 1μm.As a physical technique for achieving resolution beyond standard capabilities,this readily adaptable approach presents a powerful tool for high-definition imaging in biological research.

关 键 词：mass spectrometry imaging MALDI imaging spatial lipidomics subcellular imaging EXPANSION molecular imaging high resolution 

分 类 号：O57[理学—粒子物理与原子核物理]