Branched-chain fatty acids from goat milk alleviate ulcerative colitis via the TLR4/NF-κB/NLRP3 pathway  

作　　者：Jiaxin Zhang Jinjing Zhong Zhengying Cui Yu Shen Yaping Zheng Yu Zhang Chaoxin Man Yanmei Hou Qianyu Zhao Yujun Jiang 

机构地区：[1]Key Laboratory of Dairy Science,Ministry of Education,Department of Food Science,Northeast Agricultural University,Harbin 150030,China [2]Hyproca Nutrition Co.,Ltd.,Changsha 410000,China

出　　处：《Food Science and Human Wellness》2024年第6期3624-3632,共9页食品科学与人类健康（英文）

基　　金：financially supported by the 2021 Kabrita Nutrition Grant.

摘　　要：Branched-chain fatty acids(BCFAs)are new bioactive fatty acids with anti-inflammatory properties.However,the role of BCFAs in alleviating ulcerative colitis has not been clarified.Herein,we evaluated the protective effect of BCFAs from goat milk in mice with colitis induced using dextran sodium sulfate(DSS)and explored the corresponding mechanism.These results show that BCFAs extracted from goat milk can significantly alleviate weight loss in mice,and reduce the disease activity index and the activity of myeloperoxidase while increasing the content of antioxidant enzymes in colon tissue and reducing the oxidation stress response.These data also show that BCFAs can down-regulate the gene and protein expression of the toll-like receptor 4(TLR4)/nuclear factorκB p65(NF-κB p65)/NOD-like receptor thermal protein domain associated protein 3(NLRP3)signaling pathway,and at the same time significantly reduce the expression of pro-inflammatory factors tumor necrosis factorα(TNF-α),interleukin 1β(IL-1β),and IL-18 in colon tissue,and significantly increase the expression of the anti-inflammatory factor IL-10.In conclusion,these results demonstrated that BCFAs in goat milk exerted effects on colitis-related inflammatory cytokines and inhibited inflammation by inducing the TLR4/NF-κB/NLRP3 pathway to alleviate DSS-induced ulcerative colitis.This study provides evidence for the potential of BCFAs as bioactive fatty acids in food products and to ameliorate ulcerative colitis development in mice.

关 键 词：Goat milk Ulcerative colitis Branch-chain fatty acids TLR4/NF-κB/NLRP3 pathway 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程] R574.62[轻工技术与工程—食品科学与工程]