Genome-wide association study dissecting drought resistance-associated loci based on physiological traits in common bean  

作　　者：Lei Wu Yujie Chang Lanfen Wang Shumin Wang Jing Wu 

机构地区：[1]Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River,Ministry of Education/College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China [2]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Bejing 100081,China [3]SXAU-ICS(Shanxi Agricultural University-lnstitute of Crop Sciences)Joint Research Center of Minor Crops,Shanxi Agricultural University,Taiyuan 030031,China

出　　处：《Journal of Integrative Agriculture》2024年第11期3657-3671,共15页农业科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2019YFD1001300 and 2019YFD1001305);the Fundamental Research Funds for the Central Universities,China(SWUKQ22042);the China Agriculture Research System of MOF and MARA(CARS-08);the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences。

摘　　要：Genetic improvement of drought resistance is one of the main breeding goals for common bean,so molecular markers must be identified to facilitate drought resistance breeding.In this study,we evaluated the proline,trehalose,raffinose,and stachyose contents of 210 common bean accessions under two watering conditions and found large variations in all four.The coefficients of variation ranged from 21.21%for proline content to 78.69%for stachyose content under well-watered conditions,and from 20.11%for proline content to 50.08%for trehalose content under drought stress.According to our genome-wide association analysis,32 quantitative trait loci were associated with drought resistance,seven of which overlapped with known loci.Four hotspot regions were identified at Pv01,Pv07 and Pv11.A set of candidate genes was identified,including genes encoding MYB,bZIP,bHLH,ERF,and protein kinases.Among these genes,Phvul.001G189400,Phvul.007G273000 and Phvul.008G270500 were annotated as bZIP,ERF and WRKY,respectively.These genes are reportedly involved in drought stress responses in Arabidopsis thaliana and were induced by drought stress in common bean.Significant SNPs in six candidate gene regions formed different haplotypes,and phenotypic analysis revealed significant differences among the haplotypes.These results provide new insight into the genetic basis of drought resistance in common bean and reveal candidate genes and superior natural variations that will be useful for improving common bean.

关 键 词：common bean drought resistance GWAS physiological trait 

分 类 号：S643.1[农业科学—蔬菜学]