Emergence of a novel multi-resistance-mediating integrative and conjugative element ICEPmu3 in Pasteurella multocida  

作　　者：Jiao He Zhishuang Yang Mingshu Wang Renyong Jia Shun Chen Mafeng Liu Xinxin Zhao Qiao Yang Ying Wu Shaqiu Zhang Juan Huang Xumin Ou Di Sun Bin Tian Yu He Zhen Wu Anchun Cheng Dekang Zhu 

机构地区：[1]Research Center of Avian Diseases,College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China [2]Key Laboratory of Agricultural Bioinformatics,Ministry of Education,Chengdu 611100,China [3]International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province,Chengdu 610199,China [4]Engineering Research Center of Southwest Animal Disease Prevention and Control Technology,Ministry of Education,Chengdu 610199,China

出　　处：《Journal of Integrative Agriculture》2024年第11期3938-3942,共5页农业科学学报（英文版）

基　　金：supported by the earmarked fund for China Agriculture Research System(CARS-42-17);the Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System(SCCXTD-2021-18)。

摘　　要：Pasteurella multocida(PM)has been infecting a variety of hosts for a long time,causing sustained economic losses worldwide;however,there have been limited studies on its extensive adaptability(Aktories et al.2012).Analysis of strains data collected in our laboratory revealed that PM typically acquires foreign genes through transformation and conjugation,rather than transformation and fusion.Integrative and conjugative elements(ICEs)are a crucial mechanism that leads to abrupt changes in niche preferences and enhances environmental adaptability for bacteria,with their number far exceeding the number of plasmids and phages(Wozniak and Waldor 2010;Johnson and Grossman 2015;Botelho and Schulenburg 2021).Previous research has shown that the European bovine-sourced strain Pm36950 contains the experimentally transferable resistant ICEPmu1,which could integrate into the host chromosome or forming a circular intermediate(Michael et al.2012).Additionally,a few studies have suggested that PM strains isolated from European bovine and Asian swine might contain ICEs,but these claims lack experimental verification(Klima et al.2014;Moustafa et al.2015;Kadlec et al.2017;Peng et al.2017;Beker et al.2018;Schink et al.2022).Currently,there is no data available on ICE-carrying PM strains isolated from hosts outside of European cattle or Chinses swine.This letter presents a report on a novel ICE identified in the hypervirulent and multidrug-resistant PM HN141014 strain isolated from Chinese duck.The ICE was specifically analyzed for its resistance genes,transferable capacity and host diversity.

关 键 词：conjugative ELEMENT EXCEEDING 

分 类 号：S852.61[农业科学—基础兽医学]