针刀干预对膝骨关节炎兔软骨细胞早衰p53蛋白和HMGB1表达的影响  

Effect of Acupotomy Intervention on Expressions of p53 Protein and HMGB1 in Premature Aging Chondrocytes of KOA Rabbits

作　　者：李佳慧 张义[1] 胡芮 潘俊康 邢龙飞 胡庭尧 张晗 李宛容 郭妍 LI Jiahui;ZHANG Yi;HU Rui;PAN Junkang;XING Longfei;HU Tingyao;ZHANG Han;LI Wanrong;GUO Yan(School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China;Kunming Hospital of Traditional Chinese Medicine,Kunming 650599,China;China-Japan Friendship Hospital,Beijing 100029,China;Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University,Beijing 100010,China)

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]昆明市中医医院,云南昆明650599 [3]中日友好医院,北京100029 [4]首都医科大学附属北京中医医院,北京100010

出　　处：《针灸临床杂志》2024年第11期75-81,共7页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然科学基金青年项目,编号:82104996。

摘　　要：目的:观察针刀干预膝骨关节炎(KOA)兔对p53蛋白和HMGB1的影响。方法:24只新西兰兔随机分为4组,每组6只,为空白组、模型组、针刀组和电针组,除空白组外其他3组采用改良Videman法持续6周制作KOA模型。模型制备结束后,空白组和模型组不做处理,针刀组在患侧膝关节周围高应力点进行针刀治疗,1次/周,治疗3周。电针组取患侧血海、梁丘、内膝眼和外膝眼穴,1次/2 d,每次留针20 min,治疗3周。采用番红固绿染色法观察患侧软骨形态的病理改变,ELISA检测关节液中白细胞介素-8(IL-8)和趋化因子配体2(CCL-2)的含量,免疫组织化学检测HMGB1的表达,Western Blot法检测p53蛋白的表达水平。结果:与空白组比较,模型组软骨基质染色浅,厚度不均,软骨细胞排列杂乱无序,潮线紊乱;针刀组和电针组较模型组软骨细胞排列更规则,潮线更完整。与空白组比较,模型组兔关节液中IL-8、CCL-2水平、软骨细胞HMGB1和软骨细胞p53蛋白水平均高于空白组,差异具有统计学意义(P<0.01);与模型组比较,针刀组兔关节液中IL-8、CCL-2水平、软骨细胞HMGB1和软骨细胞p53蛋白水平均低于模型组,差异具有统计学意义(P<0.05),电针组兔关节液中IL-8、CCL-2水平、软骨细胞HMGB1和软骨细胞p53蛋白水平均低于模型组,差异具有统计学意义(P<0.05);针刀组与电针组关节液中IL-8、CCL-2水平和软骨细胞HMGB1水平差异无统计学意义(P>0.05),但针刀组p53蛋白表达水平低于电针组,差异具有统计学意义(P<0.05)。结论:针刀干预可以改善软骨细胞病理变化,延缓KOA进展,可能与降低衰老相关因子HMGB1和p53表达水平有关。Objective:To observe the effects of acupotomy on p53 protein and HMGB1 in rabbits with knee osteoarthritis(KOA).Methods:24 New Zealand rabbits were randomly divided into the blank group,the model group,the acupotomy group and the electro-acupuncture(EA)group.The KOA model was established by improved Videman method for 6 weeks.The blank group and the model group were not given any intervention,the acupotomy group was treated at the high-stress point around the affected knee joint once a week for 3 weeks.The EA group was treated with electro-needling Xuehai(SP10),Liangqiu(ST34),Neixiyan(EX-LE4)and Waixiyan(EX-LE5),once every other day for 3 weeks,with 20 minutes of needle retention each time.The pathological changes of cartilage morphology of affected side were observed by Safranin O-Fast Green staining method,the contents of interleukin-8(IL-8)and chemokine ligand 2(CCL-2)in joint fluid were detected by ELISA,the expression of HMGB1 was observed by immunohistochemistry,and the expression of p53 protein was observed by Western Blot.Results:Compared with the blank group,the model group showed shallow staining of the cartilage matrix,uneven thickness,disordered arrangement of chondrocytes,and disordered tidal lines;compared with those in the model group,the chondrocyte arrangement was more regular and the tide line was more complete in the acupotomy group and the EA group.Compared with those in the blank group,the levels of IL-8 and CCL-2 in joint fluid,as well as HMGB1 and p53 protein in chondrocytes were higher in the model group(P<0.01).The levels of IL-8 and CCL-2 in joint fluid,HMGB1 and p53 protein in chondrocytes were lower in the acupotomy group and the EA group than those in the model group(P<0.05).There were no statistical differences in the levels of IL-8 and CCL-2 in the joint fluid and HMGB1 in chondrocytes between the acupotomy group and the EA group(P>0.05);however,the expression of p53 protein was less in the acupotomy group than that in the EA group(P<0.05).Conclusion:Acupotomy intervention can impro

关 键 词：膝骨关节炎 针刀 早衰 HMGB1 P53蛋白 

分 类 号：R246.2[医药卫生—针灸推拿学]