Karyotype establishment and development of specific molecular markers of Aegilops geniculata Roth based on SLAF-seq  

作　　者：Yongfu Wang Jianzhong Fan Hong Zhang Pingchuan Deng Tingdong Li ChunhuanChen Wanquan Ji Yajuan Wang 

机构地区：[1]College of Agronomy,Northwest A&F University,Yangling 712100,China [2]State Key Laboratory of Crop Stress Biology for Arid Areas,Yangling 712100,China [3]Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement,Ministry of Agriculture and Rural Affairs,Yangling 712100,China

出　　处：《Journal of Integrative Agriculture》2024年第12期3953-3965,共13页农业科学学报（英文版）

基　　金：funded by the National Natural Science Foundation of China(31471481);the Project of Science and Technology of Shaanxi Province of China(2021NY-081 and 2023YBNY-033)。

摘　　要：The constant evolution of pathogens poses a threat to wheat resistance against diseases,endangering food security.Developing resistant wheat varieties is the most practical approach for circumventing this problem.As a close relative of wheat,Aegilops geniculata,particularly accession SY159,has evolved numerous beneficial traits that could be applied to improve wheat.In this study,we established the karyotype of SY159 by fluorescence in situ hybridization(FISH)using the oligonucleotide probes Oligo-pTa535 and Oligo-pSc119.2 and a complete set of wheat–Ae.geniculata accession TA2899 addition lines as a reference.Using specific-locus amplified fragment sequencing(SLAF-seq)technology,400 specific markers were established for detecting the SY159 chromosomes with efficiencies reaching 81.5%.The SY159-specific markers were used to classify the different homologous groups of SY159 against the wheat-Ae.geniculata addition lines.We used these specific markers on the 7Mg chromosome after classification,and successfully confirmed their suitability for studying the different chromosomes of SY159.This study provides a foundation for accelerating the application of SY159 in genetic breeding programs designed to improve wheat.

关 键 词：Aegilops geniculata Roth chromosome karyotype analysis FISH SLAF-seq specific molecular marker 

分 类 号：S512.1[农业科学—作物学]