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作 者:Xuehui Zhao Jianting Liu Xiling Fu Long Xiao Qingjie Wang Chaoran Wang ZhizhangChen Jiakui Li Changkun Lu Hui Cao Ling Li
机构地区:[1]School of Advanced Agricultural Sciences,Weifang University,Weifang 261061,China [2]College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an 271000,China [3]Development and Reform Bureau of Wendeng,Weihai 264400,China [4]Weifang Academy of Agricultural Sciences,Weifang 261061,China [5]College of Horticulture and Landscape Architecture,Yangzhou University,Yangzhou 225009,China [6]Weifang Vocational College,Weifang 261041,China [7]School of Foreign Languages,Weifang University,Weifang 261061,China
出 处:《Journal of Integrative Agriculture》2024年第12期4058-4073,共16页农业科学学报(英文版)
基 金:supported by the National Natural Science Foundation of China(32302509);the Weifang University Doctoral Research Launch Fund,China(2022BS18);the National Natural Science Foundation of China(32072518)。
摘 要:Bud dormancy is a complex physiological process of perennial woody plants living in temperate regions,and it can be affected by various phytohormones.Cytokinin oxidase/dehydrogenases(CKXs)are a group of enzymes essential for maintaining cytokinin homeostasis,yet a comprehensive analysis of these enzymes in peach remains lacking.Here,a total of 51 CKX members from different species,including six from peach,eleven from apple,nine from poplar,seven from Arabidopsis,eight from strawberry,and ten from rice,were identified using the Simple HMM Search tool of TBtools and a BLASTP program and classified into four groups using phylogenetic analysis.Conserved motif and gene structure analysis of these 51 CKX members showed that 10 conserved motifs were identified,and each CKX gene contained at least two introns.Cis-element analysis of PpCKXs showed that all PpCKX genes have light-responsive elements and at least one hormone-responsive element.The changed relative expression levels of six PpCKX genes in peach buds from endodormancy to bud-break were observed by qRT-PCR.Among them,the expression trend of PpCKX6 was almost opposite that of PpEBB1,a positive bud-break regulator in woody plants,around the bud-break stage.Y1H,EMSA,and dual-luciferase assays indicated that PpEBB1negatively regulated PpCKX6 through direct binding to a GCC box-like element located in the promoter region of PpCKX6.In addition,a transient assay showed that overexpression of PpCKX6 delayed the bud-break of peach.These results indicate that the PpCKX genes play an essential role in the dormancy-regrowth process,and Pp CKX6may act downstream of PpEBB1 directly to regulate the bud-break process,which further improves the hormoneregulatory network of dormancy-regrowth of woody plants,and provides new insights for molecular breeding and genetic engineering of peach.
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